FANCM Antibody - #DF9513
| 製品: | FANCM Antibody |
| カタログ: | DF9513 |
| タンパク質の説明: | Rabbit polyclonal antibody to FANCM |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Rabbit, Dog |
| 分子量: | 232 kDa; 232kD(Calculated). |
| ユニプロット: | Q8IYD8 |
| RRID: | AB_2842709 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9513, RRID:AB_2842709.
折りたたみ／展開
ATP-dependent RNA helicase FANCM; FAAP250; Fanconi anemia group M protein; fanconi anemia-associated polypeptide of 250 kDa; KIAA1596; Protein Hef ortholog;
免疫原
Expressed in germ cells of fetal and adult ovaries. In fetal ovaries, it is present in oogonia but expression is stronger in pachytene stage oocytes. Expressed in oocytes arrested at the diplotene stage of prophase I during the last trimester of pregnancy and in adults (PubMed:29231814). Expressed in the testis (PubMed:30075111).
- Q8IYD8 FANCM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVAAYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEIWCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFRILALSATPGSDIKAVQQVITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAIQKTYIQILESFARSLIQRNVLMRRDIPNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFAICISLYHGYELLQQMGMRSLYFFLCGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMFARTRSTSANGISAIQQGDKNKKFVYSHPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVMIFSSFRDSVQEIAEMLSQHQPIIRVMTFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTLVSTCVGEEGLDIGEVDLIICFDSQKSPIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQSQSNKRSIYKAISSNRQVLHFYQRSPRMVPDGINPKLHKMFITHGVYEPEKPSRNLQRKSSIFSYRDGMRQSSLKKDWFLSEEEFKLWNRLYRLRDSDEIKEITLPQVQFSSLQNEENKPAQESTTGIHQLSLSEWRLWQDHPLPTHQVDHSDRCRHFIGLMQMIEGMRHEEGECSYELEVESYLQMEDVTSTFIAPRNESNNLASDTFITHKKSSFIKNINQGSSSSVIESDEECAEIVKQTHIKPTKIVSLKKKVSKEIKKDQLKKENNHGIIDSVDNDRNSTVENIFQEDLPNDKRTSDTDEIAATCTINENVIKEPCVLLTECQFTNKSTSSLAGNVLDSGYNSFNDEKSVSSNLFLPFEEELYIVRTDDQFYNCHSLTKEVLANVERFLSYSPPPLSGLSDLEYEIAKGTALENLLFLPCAEHLRSDKCTCLLSHSAVNSQQNLELNSLKCINYPSEKSCLYDIPNDNISDEPSLCDCDVHKHNQNENLVPNNRVQIHRSPAQNLVGENNHDVDNSDLPVLSTDQDESLLLFEDVNTEFDDVSLSPLNSKSESLPVSDKTAISETPLVSQFLISDELLLDNNSELQDQITRDANSFKSRDQRGVQEEKVKNHEDIFDCSRDLFSVTFDLGFCSPDSDDEILEHTSDSNRPLDDLYGRYLEIKEISDANYVSNQALIPRDHSKNFTSGTVIIPSNEDMQNPNYVHLPLSAAKNEELLSPGYSQFSLPVQKKVMSTPLSKSNTLNSFSKIRKEILKTPDSSKEKVNLQRFKEALNSTFDYSEFSLEKSKSSGPMYLHKSCHSVEDGQLLTSNESEDDEIFRRKVKRAKGNVLNSPEDQKNSEVDSPLHAVKKRRFPINRSELSSSDESENFPKPCSQLEDFKVCNGNARRGIKVPKRQSHLKHVARKFLDDEAELSEEDAEYVSSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHKNINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRAVMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCLNSVPSGSSAQSKVRSTPRVNPLAKQSKQTSLNLKDTISEVSDFKPQNHNEVQSTTPPFTTVDSQKDCRKFPVPQKDGSALEDSSTSGASCSKSRPHLAGTHTSLRLPQEGKGTCILVGGHEITSGLEVISSLRAIHGLQVEVCPLNGCDYIVSNRMVVERRSQSEMLNSVNKNKFIEQIQHLQSMFERICVIVEKDREKTGDTSRMFRRTKSYDSLLTTLIGAGIRILFSSCQEETADLLKELSLVEQRKNVGIHVPTVVNSNKSEALQFYLSIPNISYITALNMCHQFSSVKRMANSSLQEISMYAQVTHQKAEEIYRYIHYVFDIQMLPNDLNQDRLKSDI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IYD8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T29 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| K400 | Acetylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| Y609 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| Y647 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| S832 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K859 | Acetylation | Uniprot | |
| K862 | Acetylation | Uniprot | |
| K867 | Acetylation | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S997 | Phosphorylation | Uniprot | |
| S1045 | Phosphorylation | Uniprot | |
| S1158 | Phosphorylation | Uniprot | |
| S1162 | Phosphorylation | Uniprot | |
| S1322 | Phosphorylation | Uniprot | |
| T1413 | Phosphorylation | Uniprot | |
| S1417 | Phosphorylation | Uniprot | |
| S1437 | Phosphorylation | Uniprot | |
| S1448 | Phosphorylation | Uniprot | |
| S1571 | Phosphorylation | Uniprot | |
| S1673 | Phosphorylation | Uniprot | |
| S1674 | Phosphorylation | Uniprot | |
| S1686 | Phosphorylation | Uniprot | |
| S1693 | Phosphorylation | Uniprot | |
| S1720 | Phosphorylation | Uniprot | |
| T1721 | Phosphorylation | Uniprot | |
| S1735 | Phosphorylation | Uniprot | |
| T1760 | Phosphorylation | Uniprot | |
| S1783 | Phosphorylation | Uniprot | |
| K1797 | Ubiquitination | Uniprot | |
| S1874 | Phosphorylation | Uniprot | |
| K1916 | Ubiquitination | Uniprot |
研究背景
DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates. In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA.
Phosphorylated; hyperphosphorylated in response to genotoxic stress.
Nucleus.
Expressed in germ cells of fetal and adult ovaries. In fetal ovaries, it is present in oogonia but expression is stronger in pachytene stage oocytes. Expressed in oocytes arrested at the diplotene stage of prophase I during the last trimester of pregnancy and in adults. Expressed in the testis.
Component of the Fanconi anemia (FA) core complex, which consists of CENPS, CENPX, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, FANCM, FAAP24 and FAAP100. The FA core complex associates with Bloom syndrome (BLM) complex, which consists of at least BLM, DNA topoisomerase 3-alpha/TOP3A, RMI1/BLAP75, RPA1/RPA70 and RPA2/RPA32. This supercomplex between FA and BLM complexes has been called BRAFT. Forms a discrete complex with CENPS and CENPX, called FANCM-MHF; this interaction stimulates DNA binding and replication fork remodeling by FANCM and stabilizes the binding partners. Forms a heterodimer with FAAP24; this interaction increases FANCM single-stranded DNA-binding activity.
Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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