製品: GAPDHS Antibody
カタログ: DF9535
タンパク質の説明: Rabbit polyclonal antibody to GAPDHS
アプリケーション: WB
反応性: Human, Monkey
予測: Zebrafish, Horse, Dog
分子量: 46 kDa,55kDa; 45kD(Calculated).
ユニプロット: O14556
RRID: AB_2842731

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Monkey
予測:
Zebrafish(89%), Horse(83%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
GAPDHS Antibody detects endogenous levels of total GAPDHS.
RRID:
AB_2842731
引用形式: Affinity Biosciences Cat# DF9535, RRID:AB_2842731.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

G3PT_HUMAN; GAPD2; GAPDH 2; GAPDH-2; GAPDH2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; Glyceraldehyde-3-phosphate dehydrogenase; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; testis-specific;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O14556 G3PT_HUMAN:

Testis specific.

タンパク質配列:
MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Zebrafish
89
Horse
83
Pig
75
Bovine
75
Rabbit
75
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14556 基板として

Site PTM Type Enzyme
T77 Phosphorylation
T301 Phosphorylation

研究背景

機能:

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis specific.

サブユニット構造:

Homotetramer. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

タンパク質ファミリー:

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

研究領域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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