G-CSF Antibody - #DF9542

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製品: G-CSF Antibody
カタログ: DF9542
タンパク質の説明: Rabbit polyclonal antibody to G-CSF
アプリケーション: WB IHC
反応性: Human, Rat
予測: Bovine, Sheep, Rabbit
分子量: 22 kDa; 22kD(Calculated).
ユニプロット: P09919
RRID: AB_2842738

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関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Bovine(83%), Sheep(83%), Rabbit(82%)
クローナリティ:
Polyclonal
特異性:
G-CSF Antibody detects endogenous levels of total G-CSF.
RRID:
AB_2842738
引用形式: Affinity Biosciences Cat# DF9542, RRID:AB_2842738.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C17orf33; Colony stimulating factor 3 (granulocyte); Colony stimulating factor 3; CSF 3; CSF beta; CSF3; CSF3_HUMAN; CSF3OS; Csfg; Filgrastim; G-CSF; GCSA; GCSF; Granulocyte colony stimulating factor; Granulocyte colony-stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin;

免疫原

免疫原:
Uniprot:

P09919(CSF3_HUMAN) >>Visit HPA database.

遺伝子(ID):
CSF3(1440)
タンパク質配列:
MAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLVSECATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Rabbit
82
Dog
73
Pig
64
Horse
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09919 基板として

Site PTM Type Enzyme
T6 Phosphorylation

研究背景

機能:

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

PTMs:

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the IL-6 superfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). Single‐cell profiling reveals distinct immune phenotypes that contribute to ischaemia‐reperfusion injury after steatotic liver transplantation. Cell Proliferation, 2021 (PubMed: 34469018) [IF=8.5]

Application: IF/ICC    Species: Rat    Sample: Kupffer cells

FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells. (A) t- SNE plots of the expression of representative marker genes for Kupffer cells. (B) UMAP plot of Kupffer cells by clusters (up) and group (down). CDL, control donor liver; FDL, fatty donor liver. (C) Violin plot showing the expression of marker genes in the indicated clusters of Kupffer cells. The colourful bars corresponding to specific clusters in (b). (D) Volcano plot shows differentially expressed genes between CDL (blue dots) and FDL Kupffer cells (red dots). The names of some significant genes are indicated in the plots. (E) The KEGG pathway enrichment analysis of differentially expressed genes (DEGs) among Kupffer cell subtypes. (F) Multiplexed immunofluorescence staining to validate the existence of CSF3 + Kupffer cells (KCs) in FDL sample, white arrows (CLEC4F + CSF3 + ). Scale bar, 20 μm. (G) Connection graph showing the intensity of interactions between one cell type to another in coloured circles. Interactions were evaluated among all cell types, including immune and non- immune fractions. The node size indicated the number of L- R interactions

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