GNL3 Antibody - #DF9566
| 製品: | GNL3 Antibody |
| カタログ: | DF9566 |
| タンパク質の説明: | Rabbit polyclonal antibody to GNL3 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 62 kDa; 62kD(Calculated). |
| ユニプロット: | Q9BVP2 |
| RRID: | AB_2842762 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9566, RRID:AB_2842762.
折りたたみ／展開
C77032; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced;
免疫原
- Q9BVP2 GNL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTVLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BVP2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Acetylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K79 | Acetylation | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K224 | Methylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| R230 | Methylation | Uniprot | |
| K237 | Acetylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K275 | Sumoylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T289 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K388 | Sumoylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K444 | Sumoylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| K534 | Sumoylation | Uniprot | |
| Y542 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot |
研究背景
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
Nucleus. Nucleus>Nucleolus.
Note: Shuttles between the nucleus and nucleolus.
Increased levels in lung tissue in cancer patients.
Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (By similarity).
The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Belongs to the TRAFAC class YlqF/YawG GTPase family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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