ACSL1 Antibody - #DF9605

Fig. 5. PM2.5 induced iron overload and lipid peroxidation in EAU. (A) Representative fluorescence images (left) and fluorescence intensity quantification (right) of FerroOrange indicating the intracellular Fe2+ content of CD4+ T cells in EUA mice with or without PM2.5 exposure (n=6/ group; unpaired student’s t-test; scale bar: 25μm). (B) Representative flow cytometry images (left) and quantification (right) of ROS level in CD4+ T cells of EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (C) Representative flow cytometry images (left) and quantification (right) of oxidized and non-oxidized lipid production of CD4+ T cells in EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (D) The concentration of MDA in serum of EAU mice with or without PM2.5 exposure (n=3/ group; unpaired student’s t-test). (E) The concentration of MDA in CD4+ T cells of EAU mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (F) GSH/GSSH ratio in CD4+ T cells of EUA mice with or without PM2.5 exposure (n=5/ group; unpaired student’s t-test). (G) Representative Western blotting images of TFRC, FTH1, FTL1, ACSL1, HMOX1, GPX4 and β-actin in vivo (left) and in vitro (right). (H) Quantification of relative expression of TFRC, FTH1, FTL1, ACSL1, HMOX1, GPX4 in vivo and in vitro (n=3/ group; one-way ANOVA). β-actin served as an internal control. Data represent mean ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Fig. 3 Antagomir-103-3p alleviated the damage to mice with NAFLD. Mice with NAFLD were fed an HFD for 8 weeks, and Antagomir-NC or Antagomir-103-3p was used for tail vein injection once a week for 2 weeks. A MiR-103-3p expression in mouse liver tissues was examined by qRT-PCR. B Oil Red O staining detected lipid droplet accumulation in mouse liver tissues, and HE staining detected liver tissue lesions in mice. C The TG, ALT, AST and H2O2 contents in mouse serum were examined, while ROS generation and ATP content were examined in mouse tissues. D The protein and mRNA levels of ACOX1, FASN and ACSL1 were examined by western blotting and qRT-PCR, respectively. *P < 0.05 compared with the control group; #P < 0.05 compared with the NAFLD+Antagomir-NC group