MED22 Antibody - #DF9614
| 製品: | MED22 Antibody |
| カタログ: | DF9614 |
| タンパク質の説明: | Rabbit polyclonal antibody to MED22 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 22 kDa; 22kD(Calculated). |
| ユニプロット: | Q15528 |
| RRID: | AB_2842810 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9614, RRID:AB_2842810.
折りたたみ／展開
MED22; MED22_HUMAN; MED24; Mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22; OTTHUMP00000022480; OTTMUSP00000012503; RGD1564893; Surf 5; Surf-5; Surfeit 5; Surfeit locus protein 5;
免疫原
- Q15528 MED22_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSSSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15528 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| T107 | Phosphorylation | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Nucleus.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
Belongs to the Mediator complex subunit 22 family.
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