NDUFC1 Antibody - #DF9667

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製品: NDUFC1 Antibody
カタログ: DF9667
タンパク質の説明: Rabbit polyclonal antibody to NDUFC1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit
分子量: 9kDa; 9kD(Calculated).
ユニプロット: O43677
RRID: AB_2842863

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Horse(91%), Rabbit(91%)
クローナリティ:
Polyclonal
特異性:
NDUFC1 Antibody detects endogenous levels of total NDUFC1.
RRID:
AB_2842863
引用形式: Affinity Biosciences Cat# DF9667, RRID:AB_2842863.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CI-KFYI; Complex I KFYI subunit; Complex I-KFYI; KFYI; mitochondrial; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa; NADH dehydrogenase [ubiquinone] 1 subunit C1; NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; NADH-ubiquinone oxidoreductase KFYI subunit; NDUC1_HUMAN; NDUFC1;

免疫原

免疫原:
Uniprot:

O43677(NDUC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
NDUFC1(4717)
タンパク質配列:
MAPSALLRPLSRLLAPARLPSGPSVRSKFYVREPPNAKPDWLKVGFTLGTTVFLWIYLIKQHNEDILEYKRRNGLE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Rabbit
91
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43677 基板として

Site PTM Type Enzyme
K70 Ubiquitination

研究背景

機能:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

細胞の位置付け:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Complex I is composed of 45 different subunits.

タンパク質ファミリー:

Belongs to the complex I NDUFC1 subunit family.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

参考文献

1). Urolithin A protects severe acute pancreatitis-associated acute cardiac injury by regulating mitochondrial fatty acid oxidative metabolism in cardiomyocytes. MedComm, 2023 (PubMed: 38116065) [IF=9.9]

Application: WB    Species: Mouse    Sample: cardiac tissues

FIGURE 2 Effect of Urolithin A on mitochondria‐related genes in SACI mice. (A) Venn diagram of gene expression in sham group vs. SACI group and SACI group vs. UA group. (B) Heatmap analysis of 90 overlapping gene expressions in three groups (n = 4). (C) GO term analysis of 90 overlapping gene expressions in the SACI group vs. UA group. (D) Volcano plots of differential genes in the sham group vs. SACI group and UA group vs. SACI group. (E) Western blotting and quantitative analysis of COX I, COX IV, TOMM7, and NDUFC1 in cardiac tissues of the sham, SACI, and UA groups (n = 3). (F) Representative transmission electron microscopy (TEM) images of cardiac tissues in the sham, SACI, and UA groups (scale bar = 2 μm; 500 nm). (G) Mitochondrial membrane potential changes in isolated purified heart mitochondria in the sham, SACI, and UA groups. (JC‐1 fluorescence: red/green ratio; n = 4). (H) Changes in total ATP concentration in heart tissues in the sham, SACI, and UA groups (n = 6). The results are presented as the mean ± SD. **p < 0.01, ***p < 0.005, ****p < 0.0001 vs. sham group; ##p < 0.01, ###p < 0.005, ####p < 0.0001 vs. SACI group, by one‐way ANOVA tests followed by Tukey tests.

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