NUP133 Antibody - #DF9706
(1)
| 製品: | NUP133 Antibody |
| カタログ: | DF9706 |
| タンパク質の説明: | Rabbit polyclonal antibody to NUP133 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 129 kDa; 129kD(Calculated). |
| ユニプロット: | Q8WUM0 |
| RRID: | AB_2842901 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(90%), Zebrafish(89%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(100%), Chicken(90%)
クローナリティ:
Polyclonal
特異性:
NUP133 Antibody detects endogenous levels of total NUP133.
RRID:
AB_2842901
引用形式: Affinity Biosciences Cat# DF9706, RRID:AB_2842901.
引用形式: Affinity Biosciences Cat# DF9706, RRID:AB_2842901.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
133 kDa nucleoporin; FLJ10814; hNUP 133; hNUP133; MGC21133; NU133_HUMAN; Nuclear pore complex protein Nup133; Nucleoporin 133kDa; Nucleoporin NUP133; NUP 133; NUP133; OTTHUMP00000061095;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質配列:
- Q8WUM0 NU133_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDLKQNCDGLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLYNESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Dog
100
Pig
90
Horse
90
Bovine
90
Sheep
90
Chicken
90
Rabbit
90
Zebrafish
89
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WUM0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| R9 | Methylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| R17 | Methylation | Uniprot | |
| S27 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T28 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T31 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S56 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| R61 | Methylation | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| K82 | Sumoylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| T134 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| T512 | Phosphorylation | Uniprot | |
| K518 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| K758 | Acetylation | Uniprot | |
| K758 | Ubiquitination | Uniprot | |
| Y762 | Phosphorylation | Uniprot | |
| K787 | Ubiquitination | Uniprot | |
| Y832 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| K913 | Ubiquitination | Uniprot | |
| T961 | Phosphorylation | Uniprot | |
| K967 | Ubiquitination | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| T969 | Phosphorylation | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| S1021 | Phosphorylation | Uniprot | |
| T1027 | Phosphorylation | Uniprot | |
| Y1046 | Phosphorylation | Uniprot | |
| K1049 | Ubiquitination | Uniprot | |
| K1076 | Ubiquitination | Uniprot | |
| S1084 | Phosphorylation | Uniprot | |
| S1085 | Phosphorylation | Uniprot | |
| S1086 | Phosphorylation | Uniprot | |
| K1089 | Ubiquitination | Uniprot | |
| K1097 | Ubiquitination | Uniprot | |
| K1107 | Ubiquitination | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| K1123 | Ubiquitination | Uniprot | |
| S1133 | Phosphorylation | Uniprot | |
| K1135 | Ubiquitination | Uniprot | |
| Y1150 | Phosphorylation | Uniprot |
研究背景
機能:
Involved in poly(A)+ RNA transport. Involved in nephrogenesis.
細胞の位置付け:
Nucleus>Nuclear pore complex. Chromosome>Centromere>Kinetochore.
Note: Located on both the cytoplasmic and nuclear sides of the nuclear pore (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755).
組織特異性:
Widely expressed in fetal and adult tissues. Expressed in the brain and kidney.
サブユニット構造:
Forms part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus.
タンパク質ファミリー:
Belongs to the nucleoporin Nup133 family.
研究領域
· Genetic Information Processing > Translation > RNA transport.
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