SLC22A4 Antibody - #DF9724

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製品: SLC22A4 Antibody
カタログ: DF9724
タンパク質の説明: Rabbit polyclonal antibody to SLC22A4
アプリケーション: WB
反応性: Human, Mouse
分子量: 62 kDa; 62kD(Calculated).
ユニプロット: Q9H015
RRID: AB_2842919

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
SLC22A4 Antibody detects endogenous levels of total SLC22A4.
RRID:
AB_2842919
引用形式: Affinity Biosciences Cat# DF9724, RRID:AB_2842919.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Ergothioneine transporter; ET transporter; integral membrane transport protein; LSTP Like 2; MGC34546; MGC40524; Octn1; Organic cation transporter 1; Organic cation/carnitine transporter 1; RP23-354D12.1; S22A4_HUMAN; Slc22a4; Solute carrier family (organic cation transporter), member 4; solute carrier family 22 (organic cation/zwitterion transporter), member 4; Solute carrier family 22 member 4;

免疫原

免疫原:
Uniprot:

Q9H015(S22A4_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A4(6583)
発現特異性:
Q9H015 S22A4_HUMAN:

Widely expressed. Highly expressed in whole blood, bone marrow, trachea and fetal liver. Weakly expressed in kidney, skeletal muscle, prostate, lung, pancreas, placenta, heart, uterus, spleen and spinal cord. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells. Predominantly expressed in CD14 cells in peripheral blood mononuclear cells.

タンパク質配列:
MRDYDEVIAFLGEWGPFQRLIFFLLSASIIPNGFNGMSVVFLAGTPEHRCRVPDAANLSSAWRNNSVPLRLRDGREVPHSCSRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTVVTEWNLVCEDNWKVPLTTSLFFVGVLLGSFVSGQLSDRFGRKNVLFATMAVQTGFSFLQIFSISWEMFTVLFVIVGMGQISNYVVAFILGTEILGKSVRIIFSTLGVCTFFAVGYMLLPLFAYFIRDWRMLLLALTVPGVLCVPLWWFIPESPRWLISQRRFREAEDIIQKAAKMNNIAVPAVIFDSVEELNPLKQQKAFILDLFRTRNIAIMTIMSLLLWMLTSVGYFALSLDAPNLHGDAYLNCFLSALIEIPAYITAWLLLRTLPRRYIIAAVLFWGGGVLLFIQLVPVDYYFLSIGLVMLGKFGITSAFSMLYVFTAELYPTLVRNMAVGVTSTASRVGSIIAPYFVYLGAYNRMLPYIVMGSLTVLIGILTLFFPESLGMTLPETLEQMQKVKWFRSGKKTRDSMETEENPKVLITAF

PTMs - Q9H015 基板として

Site PTM Type Enzyme
T88 Phosphorylation
S93 Phosphorylation
S468 Phosphorylation

研究背景

機能:

Sodium-ion dependent, low affinity carnitine transporter. Probably transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 1.78. A key substrate of this transporter seems to be ergothioneine (ET).

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Highly expressed in whole blood, bone marrow, trachea and fetal liver. Weakly expressed in kidney, skeletal muscle, prostate, lung, pancreas, placenta, heart, uterus, spleen and spinal cord. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells. Predominantly expressed in CD14 cells in peripheral blood mononuclear cells.

サブユニット構造:

Interacts with PDZK1.

タンパク質ファミリー:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

研究領域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

参考文献

1). Cigarette smoke extract combined with lipopolysaccharide reduces OCTN1/2 expression in human alveolar epithelial cells in vitro and rat lung in vivo under inflammatory conditions. International Immunopharmacology, 2020 (PubMed: 32707498) [IF=5.6]

Application: WB    Species: human    Sample: A549 cells

Fig. 6. |The protein expression of OCTN1/2 and inflammatory factors in A549 cells at different concentrations. (A) The expression of OCTN1/2, IL-6, TNF-α, and IL1β in A549 cells was determined by western blot.
2). LPS-induced inflammation delays the transportation of ASP+ due to down-regulation of OCTN1/2 in alveolar epithelial cells. JOURNAL OF DRUG TARGETING, 2020 (PubMed: 31591905) [IF=4.5]

Application: WB    Species: human    Sample: A549 cells

Fig.2.| LPS regulates the level of OCTN1/2 and inflammation in a non-dose dependent manner in A549 cells. After cells were induced by different concentrations of LPS (0, 2, 10, 50, and 250 μg/mL) for 24 h, the expression of OCTN1 (62 kDa), OCTN2 (63 kDa), IL6 (24 kDa), IL18 (26 kDa), IL1β (31 kDa),and TNF-α (26 kDa) was significantly regulated in human respiratory epithelial cells;β-actin (42 kDa) was used as a loading control. Bands were analysed using ImageJ 1.45s (National Institutes of Health, USA). NC indicates the normal control groups. Data are shown as the mean ± SD (n = 3).

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