ATP2B2 Antibody - #DF9757

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製品: ATP2B2 Antibody
カタログ: DF9757
タンパク質の説明: Rabbit polyclonal antibody to ATP2B2
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 137 kDa; 137kD(Calculated).
ユニプロット: Q01814
RRID: AB_2842952

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ATP2B2 Antibody detects endogenous levels of total ATP2B2.
RRID:
AB_2842952
引用形式: Affinity Biosciences Cat# DF9757, RRID:AB_2842952.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AT2B2_HUMAN; atp2b2; ATPase plasma membrane Ca2+ transporting 2; Plasma membrane calcium ATPase 2; Plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium transporting ATPase 2; Plasma membrane calcium-transporting ATPase 2; PMCA2; PMCA2a; PMCA2i;

免疫原

免疫原:
Uniprot:

Q01814(AT2B2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ATP2B2(491)
発現特異性:
Q01814 AT2B2_HUMAN:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

タンパク質配列:
MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
73
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01814 基板として

Site PTM Type Enzyme
T5 Phosphorylation
S18 Phosphorylation
K177 Ubiquitination
S184 Phosphorylation
K301 Acetylation
S346 Phosphorylation
K383 Ubiquitination
K389 Acetylation
K389 Ubiquitination
T449 Phosphorylation
Y469 Phosphorylation
S470 Phosphorylation
K476 Ubiquitination
S498 Phosphorylation
S528 Phosphorylation
Y597 Phosphorylation
S604 Phosphorylation
T726 Phosphorylation
K770 Ubiquitination
K780 Ubiquitination
S792 Phosphorylation
T794 Phosphorylation
K796 Ubiquitination
T798 Phosphorylation
T818 Phosphorylation
T822 Phosphorylation
K829 Ubiquitination
Y940 Phosphorylation
K944 Ubiquitination
R1097 Methylation
T1139 Phosphorylation
S1149 Phosphorylation
S1150 Phosphorylation
Y1152 Phosphorylation
K1157 Ubiquitination
S1160 Phosphorylation
T1162 Phosphorylation
S1178 Phosphorylation
S1211 Phosphorylation
S1229 Phosphorylation
S1230 Phosphorylation
S1231 Phosphorylation
S1234 Phosphorylation
S1238 Phosphorylation
T1241 Phosphorylation
S1242 Phosphorylation

研究背景

機能:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

細胞の位置付け:

Cell junction>Synapse. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

サブユニット構造:

Interacts with PDZD11.

タンパク質ファミリー:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

参考文献

1). Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments. Fitoterapia, 2024 (PubMed: 38580032) [IF=3.4]

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