ATP2B1/2/3 Antibody - #DF9758

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製品: ATP2B1/2/3 Antibody
カタログ: DF9758
タンパク質の説明: Rabbit polyclonal antibody to ATP2B1/2/3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 136kDa; 134kD,135kD,137kD(Calculated).
ユニプロット: Q16720 | P20020 | Q01814
RRID: AB_2842953

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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ATP2B1/2/3 Antibody detects endogenous levels of total ATP2B1/2/3.
RRID:
AB_2842953
引用形式: Affinity Biosciences Cat# DF9758, RRID:AB_2842953.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AT2B1_HUMAN; ATP2B1; ATP2B2; ATP2B3; ATP2B4; ATPase Ca++ transporting plasma membrane 1; ATPase Ca++ transporting plasma membrane 2; ATPase Ca++ transporting plasma membrane 3; ATPase Ca++ transporting plasma membrane 4; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium pump isoform 1; Plasma membrane calcium transporting ATPase 1; Plasma membrane calcium transporting ATPase 2; Plasma membrane calcium transporting ATPase 3; Plasma membrane calcium transporting ATPase 4; Plasma membrane calcium-transporting ATPase 1; PMCA1; PMCA2; PMCA3; PMCA4; AT2B1_HUMAN; ATP2B1; ATPase Ca++ transporting plasma membrane 1; Plasma membrane calcium ATPase 1; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium pump isoform 1; Plasma membrane calcium transporting ATPase 1; Plasma membrane calcium-transporting ATPase 1; PMCA 1; PMCA1; AT2B2_HUMAN; atp2b2; ATPase plasma membrane Ca2+ transporting 2; Plasma membrane calcium ATPase 2; Plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium transporting ATPase 2; Plasma membrane calcium-transporting ATPase 2; PMCA2; PMCA2a; PMCA2i;

免疫原

免疫原:
Uniprot:

Q16720(AT2B3_HUMAN) >>Visit HPA database.

P20020(AT2B1_HUMAN) >>Visit HPA database.

Q01814(AT2B2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ATP2B3(492) | ATP2B1(490) | ATP2B2(491)
発現特異性:
Q16720 AT2B3_HUMAN:

Highly expressed in the cerebellum, particulary in the presynaptic terminals of parallel fibers-Purkinje neurons. Isoform XE and isoform XB are the most abundant isoforms and are detected at low levels in brain and fetal skeletal muscle. The other isoforms are only found at lower levels and not in fetal tissues.

P20020 AT2B1_HUMAN:

Isoform B: Ubiquitously expressed. Isoform C: Found in brain cortex, skeletal muscle and heart muscle. Isoform D: Has only been found in fetal skeletal muscle. Isoform K: Found in small intestine and liver. Abundantly expressed in the endometrial epithelial cells and glandular epithelial cells in early-proliferative phase and early-secretory phases (PubMed:21400627).

Q01814 AT2B2_HUMAN:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

タンパク質配列:
MGDMANSSIEFHPKPQQQRDVPQAGGFGCTLAELRTLMELRGAEALQKIEEAYGDVSGLCRRLKTSPTEGLADNTNDLEKRRQIYGQNFIPPKQPKTFLQLVWEALQDVTLIILEVAAIVSLGLSFYAPPGEESEACGNVSGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRNGQLLQVPVAALVVGDIAQVKYGDLLPADGVLIQANDLKIDESSLTGESDHVRKSADKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGKQQDGAMESSQTKAKKQDGAVAMEMQPLKSAEGGEMEEREKKKANAPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFVIETFVVEGRTWLAECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQSYLGDTHYKEIPAPSALTPKILDLLVHAISINSAYTTKILPPEKEGALPRQVGNKTECALLGFVLDLKRDFQPVREQIPEDKLYKVYTFNSVRKSMSTVIRMPDGGFRLFSKGASEILLKKCTNILNSNGELRGFRPRDRDDMVRKIIEPMACDGLRTICIAYRDFSAGQEPDWDNENEVVGDLTCIAVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIQPGEDFLCLEGKEFNRRIRNEKGEIEQERLDKVWPKLRVLARSSPTDKHTLVKGIIDSTTGEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLLRKPYGRDKPLISRTMMKNILGHAVYQLAIIFTLLFVGELFFDIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFSNPIFCTIVLGTFGIQIVIVQFGGKPFSCSPLSTEQWLWCLFVGVGELVWGQVIATIPTSQLKCLKEAGHGPGKDEMTDEELAEGEEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESKTSIHNFMATPEFLINDYTHNIPLIDDTDVDENEERLRAPPPPSPNQNNNAIDSGIYLTTHVTKSATSSVFSSSPGSPLHSVETSL

MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGLLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSATSSSPGSPLHSLETSL

MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16720/P20020/Q01814 基板として

Site PTM Type Enzyme
K180 Ubiquitination
S187 Phosphorylation
K194 Ubiquitination
T277 Phosphorylation
S283 Phosphorylation
S336 Phosphorylation
K357 Ubiquitination
K363 Acetylation
K363 Ubiquitination
T423 Phosphorylation
Y443 Phosphorylation
S444 Phosphorylation
K450 Ubiquitination
S472 Phosphorylation
T543 Phosphorylation
S578 Phosphorylation
R580 Methylation
S584 Phosphorylation
T585 Phosphorylation
T700 Phosphorylation
K744 Ubiquitination
S766 Phosphorylation
T768 Phosphorylation
K770 Ubiquitination
T772 Phosphorylation
T792 Phosphorylation
T796 Phosphorylation
K803 Ubiquitination
Y1126 Phosphorylation
Site PTM Type Enzyme
G2 Acetylation
S8 Phosphorylation
Y11 Phosphorylation
S17 Phosphorylation
K19 Ubiquitination
K48 Ubiquitination
Y53 Phosphorylation
Y57 Phosphorylation
K62 Ubiquitination
K64 Ubiquitination
K179 Ubiquitination
S186 Phosphorylation
K193 Ubiquitination
Y220 Phosphorylation
K237 Ubiquitination
S242 Phosphorylation
K251 Ubiquitination
S253 Phosphorylation
K256 Ubiquitination
S262 Phosphorylation
T276 Phosphorylation
S282 Phosphorylation
K308 Acetylation
K311 Acetylation
K312 Ubiquitination
K324 Ubiquitination
K337 Ubiquitination
S338 Phosphorylation
K347 Ubiquitination
K359 Ubiquitination
K365 Acetylation
K365 Ubiquitination
T425 Phosphorylation
Y445 Phosphorylation
S446 Phosphorylation
K452 Ubiquitination
S474 Phosphorylation
T477 Phosphorylation
T479 Phosphorylation
T486 Phosphorylation
K533 Ubiquitination
S580 Phosphorylation
K590 Ubiquitination
K622 Ubiquitination
K634 Ubiquitination
T703 Phosphorylation
K721 Ubiquitination
K737 Ubiquitination
K747 Ubiquitination
K757 Ubiquitination
S769 Phosphorylation
T771 Phosphorylation
K773 Ubiquitination
T775 Phosphorylation
K778 Ubiquitination
T795 Phosphorylation
T799 Phosphorylation
K806 Ubiquitination
Y917 Phosphorylation
K921 Ubiquitination
K1070 Ubiquitination
K1078 Ubiquitination
T1116 Phosphorylation
S1126 Phosphorylation
S1127 Phosphorylation
Y1129 Phosphorylation
K1134 Ubiquitination
S1137 Phosphorylation
S1139 Phosphorylation
S1140 Phosphorylation
S1155 Phosphorylation
T1165 Phosphorylation
K1174 Ubiquitination
S1177 Phosphorylation
S1178 Phosphorylation
S1182 Phosphorylation
S1192 Phosphorylation
T1197 Phosphorylation
S1207 Phosphorylation
S1208 Phosphorylation
S1211 Phosphorylation
S1215 Phosphorylation
T1218 Phosphorylation
S1219 Phosphorylation
Site PTM Type Enzyme
T5 Phosphorylation
S18 Phosphorylation
K177 Ubiquitination
S184 Phosphorylation
K301 Acetylation
S346 Phosphorylation
K383 Ubiquitination
K389 Acetylation
K389 Ubiquitination
T449 Phosphorylation
Y469 Phosphorylation
S470 Phosphorylation
K476 Ubiquitination
S498 Phosphorylation
S528 Phosphorylation
Y597 Phosphorylation
S604 Phosphorylation
T726 Phosphorylation
K770 Ubiquitination
K780 Ubiquitination
S792 Phosphorylation
T794 Phosphorylation
K796 Ubiquitination
T798 Phosphorylation
T818 Phosphorylation
T822 Phosphorylation
K829 Ubiquitination
Y940 Phosphorylation
K944 Ubiquitination
R1097 Methylation
T1139 Phosphorylation
S1149 Phosphorylation
S1150 Phosphorylation
Y1152 Phosphorylation
K1157 Ubiquitination
S1160 Phosphorylation
T1162 Phosphorylation
S1178 Phosphorylation
S1211 Phosphorylation
S1229 Phosphorylation
S1230 Phosphorylation
S1231 Phosphorylation
S1234 Phosphorylation
S1238 Phosphorylation
T1241 Phosphorylation
S1242 Phosphorylation

研究背景

機能:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in the cerebellum, particulary in the presynaptic terminals of parallel fibers-Purkinje neurons. Isoform XE and isoform XB are the most abundant isoforms and are detected at low levels in brain and fetal skeletal muscle. The other isoforms are only found at lower levels and not in fetal tissues.

サブユニット構造:

Interacts with PDZD11.

タンパク質ファミリー:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

機能:

Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane. Cell junction>Synapse.
Note: Colocalizes with SV2A in photoreceptor synaptic terminals. Colocalizes with NPTN to the immunological synapse. Colocalizes with EPB41 to the basolateral membrane in enterocyte.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform B: Ubiquitously expressed. Isoform C: Found in brain cortex, skeletal muscle and heart muscle. Isoform D: Has only been found in fetal skeletal muscle. Isoform K: Found in small intestine and liver. Abundantly expressed in the endometrial epithelial cells and glandular epithelial cells in early-proliferative phase and early-secretory phases.

サブユニット構造:

Monomer. Dimer. Oligomer. Calmodulin binding. Interacts with PDZD11. Interacts with SLC35G1 and STIM1; inhibits calcium-transporting ATPase activity after store depletion. Interacts with YWHAE; interacts with the monomeric and dimeric forms of the YWHAE but prefer the monomer form; this interaction inhibits calcium-transporting ATPase activity. Interacts with NPTN; this interaction stabilizes ATP2B1 and increases ATPase activity; this interaction controls T cell calcium homeostasis following T cell activation. Interacts with EPB41; regulates small intestinal calcium absorption through regulation of membrane expression of ATP2B1 (By similarity).

タンパク質ファミリー:

Isoforms A, C, D and E contain an additional calmodulin-binding subdomain B which is different in the different splice variants and shows pH dependent calmodulin binding properties.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

機能:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

細胞の位置付け:

Cell junction>Synapse. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

サブユニット構造:

Interacts with PDZD11.

タンパク質ファミリー:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Mineral absorption.

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