RBM16 Antibody - #DF9790
| 製品: | RBM16 Antibody |
| カタログ: | DF9790 |
| タンパク質の説明: | Rabbit polyclonal antibody to RBM16 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 141kDa; 141kD(Calculated). |
| ユニプロット: | Q9UPN6 |
| RRID: | AB_2842985 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9790, RRID:AB_2842985.
折りたたみ／展開
CDC5L complex associated protein 7; CDC5L complex-associated protein 7; Protein SCAF8; putative RNA binding protein 16; RBM 16; RNA binding motif protein 16; RNA binding protein 16; RNA-binding motif protein 16; SCAF 8; SCAF8; SCAF8_HUMAN; SR like CTD associated factor 8; SR related and CTD associated factor 8; SR-related and CTD-associated factor 8;
免疫原
- Q9UPN6 SCAF8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAVKTFNSELYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIVRQSRHQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPPPVVTPVLASTTTAMSNTPGTPVTPVTPANVVQGLPDPWVSQITNTDTLAAVAQILQSPQGQQLQQLIQTLQIQQQKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLEQGVSFNKKLMDRFDFGEDSEHSEEPKKEIPASQLSHVSESVNNSIFHQIAEQLQQQNLEHLRQQLLEQQQPQKATPQDSQEGTFGSEHSASPSQGSSQQHFLEPEVNLDDSIDIQQQDMDIDEGQDGVEEEVFEQEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRSGSRKRKHRKRSRSRSRERKRKSSRSYSSERRAREREKERQKKGLPPIRSKTLSVCSTTLWVGQVDKKATQQDLTNLFEEFGQIESINMIPPRGCAYVCMVHRQDAFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQFWDVDLGVTYIPWEKVKVDDLEGFAEGGMIDQETVNTEWETVKSSEPVKETVQTTQSPTPVEKETVVTTQAEVFPPPVAMLQIPVAPAVPTVSLVPPAFPVSMPVPPPGFSPIPPPPFLRASFNPSQPPPGFMPPPVPPPVVPPPTIPPVVPTSLVQPSLSMTPETVKDVGFGSLVIPGGSVASNLATSALPAGNVFNAPTKQAEPEEKVPHLIDHQISSGENTRSVIPNDISSNAAILGGQPPNVTSNSGILGVQRPNVSSNSEILGVRPSNVSSSSGIIAAQPPNILNNSGILGIQPPSVSNSSGLLGVLPPNIPNNSGLVGVQPPNVPNTPGLLGTQPPAGPQNLPPLSIPNQRMPTMPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UPN6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K18 | Sumoylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K461 | Acetylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| K609 | Acetylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| T614 | O-Glycosylation | Uniprot | |
| T615 | O-Glycosylation | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| T619 | Phosphorylation | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| R917 | Methylation | Uniprot | |
| R927 | Methylation | Uniprot | |
| R938 | Methylation | Uniprot | |
| R967 | Methylation | Uniprot | |
| R981 | Methylation | Uniprot | |
| R988 | Methylation | Uniprot | |
| R1062 | Methylation | Uniprot | |
| R1073 | Methylation | Uniprot | |
| K1091 | Ubiquitination | Uniprot | |
| R1120 | Methylation | Uniprot | |
| R1122 | Methylation | Uniprot | |
| R1124 | Methylation | Uniprot | |
| R1129 | Methylation | Uniprot | |
| R1133 | Methylation | Uniprot | |
| R1139 | Methylation | Uniprot | |
| Y1198 | Phosphorylation | Uniprot | |
| Y1237 | Phosphorylation | Uniprot |
研究背景
Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.
Nucleus. Nucleus matrix.
Note: Detected in granular nuclear foci which correspond to sites of active transcription.
Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5'. Identified in a complex with CDC5L and other spliceosomal proteins.
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