RRAGD Antibody - #DF9812
| 製品: | RRAGD Antibody |
| カタログ: | DF9812 |
| タンパク質の説明: | Rabbit polyclonal antibody to RRAGD |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 46 kDa; 46kD(Calculated). |
| ユニプロット: | Q9NQL2 |
| RRID: | AB_2843006 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9812, RRID:AB_2843006.
折りたたみ／展開
bA11D8.2.1; DKFZp761H171; FLJ44176; OTTHUMP00000016853; Rag D; Rag D protein; RagD; RagDRRAGD; Ras related GTP binding D; Ras-related GTP binding protein D; Ras-related GTP-binding protein D; RRAGD; RRAGD_HUMAN;
免疫原
- Q9NQL2 RRAGD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQL2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S76 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| T391 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Cytoplasm. Nucleus. Lysosome.
Note: Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA (PubMed:11073942).
Forms a heterodimer with RRAGA in a sequence-independent manner and RRAGB. Heterodimerization stabilizes RRAG proteins. In complex with RRAGB, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGD and is negatively regulated by amino acids Ref.14). The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor. Interacts with SESN1, SESN2 AND SESN3.
Belongs to the GTR/RAG GTP-binding protein family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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