RAB1A Antibody - #DF9817
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9817, RRID:AB_2843011.
折りたたみ／展開
GTP binding protein RAB 1A; mKIAA3012; RAB 1; Rab 1A; RAB1; RAB1, member RAS oncogene family; Rab1A; RAB1A member RAS oncogene family; RAB1A_HUMAN; Ras related protein Rab 1A; Ras-associated protein RAB1; Ras-related protein Rab-1A; YPT1; YPT1 related protein; YPT1-related protein;
免疫原
- P62820 RAB1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P62820 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| Y10 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K61 | Acetylation | Uniprot | |
| T67 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| R111 | Methylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| C126 | S-Nitrosylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot |
研究背景
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
Phosphorylated by CDK1 kinase during mitosis.
Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.
Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm>Cytosol. Membrane. Melanosome.
Note: Alternates between membrane-associated and cytosolic forms.
May interact with YIPF5 (By similarity). Interacts with C9orf72; the interaction mediates recruitment of RAB1A to the ATG1/ULK1 kinase complex. Interacts with GDI1; this promotes dissociation from membranes.
(Microbial infection) Interacts with L.pneumophila AnkX.
(Microbial infection) Interacts with L.pneumophila Lem3.
(Microbial infection) Interacts with L.pneumophila LidA.
(Microbial infection) Interacts with L.pneumophila DrrA; this disrupts the interaction between RAB1A and GDI1 and promotes the exchange of RAB1A-bound GDP with GTP.
(Microbial infection) Interacts with E.coli EspG and S.flexneri VirA; this impairs ER to Golgi trafficking and protein secretion.
(Microbial infection) Interacts with L.pneumophila and L.drancourtii LepB; this enhances RAB1A GTPase activity.
(Microbial infection) Identified in a complex composed of RAB1A, ARF6 and E.coli EspG.
Belongs to the small GTPase superfamily. Rab family.
研究領域
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
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