RAB5B Antibody - #DF9834
| 製品: | RAB5B Antibody |
| カタログ: | DF9834 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAB5B |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 24 kDa; 24kD(Calculated). |
| ユニプロット: | P61020 |
| RRID: | AB_2843028 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9834, RRID:AB_2843028.
折りたたみ／展開
Rab 5b; Rab5 b; RAB5B, member RAS oncogene family; Ras related protein Rab 5B; RAS-associated protein RAB5B;
免疫原
- P61020 RAB5B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61020 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| T6 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| K17 | Ubiquitination | Uniprot | |
| K22 | Acetylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S125 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot |
研究背景
Protein transport. Probably involved in vesicular traffic (By similarity).
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome membrane>Lipid-anchor. Melanosome.
Note: Enriched in stage I melanosomes.
Binds EEA1. Interacts with RIN2 and RIN3, which probably regulate its pathway, possibly by acting as GEFs. Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Ser-84 disrupts this interaction.
Belongs to the small GTPase superfamily. Rab family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
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