RAB8A Antibody - #DF9836
製品: | RAB8A Antibody |
カタログ: | DF9836 |
タンパク質の説明: | Rabbit polyclonal antibody to RAB8A |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat, Monkey |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
分子量: | 24 kDa; 24kD(Calculated). |
ユニプロット: | P61006 |
RRID: | AB_2843030 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9836, RRID:AB_2843030.
折りたたみ/展開
AA409338; MEL; Mel transforming oncogene (derived from cell line NK14) RAB8 homolog; Mel transforming oncogene (derived from cell line NK14); Mel transforming oncogene (RAB8 homolog); Mel transforming oncogene; MGC124948; Oncogene c mel; Oncogene c-mel; RAB8; RAB8A; RAB8A member RAS oncogene family; RAB8A_HUMAN; Ras associated protein RAB8; Ras related protein Rab 8A; Ras-related protein Rab-8A;
免疫原
- P61006 RAB8A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61006 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K3 | Acetylation | Uniprot | |
K3 | Ubiquitination | Uniprot | |
T4 | Phosphorylation | Uniprot | |
Y5 | Phosphorylation | Uniprot | |
Y7 | Phosphorylation | Uniprot | |
S17 | Phosphorylation | Uniprot | |
T49 | Phosphorylation | Uniprot | |
T64 | Phosphorylation | Uniprot | |
T72 | Phosphorylation | Uniprot | |
T74 | Phosphorylation | Uniprot | |
Y77 | Phosphorylation | Uniprot | |
Y78 | Phosphorylation | Uniprot | |
S111 | Phosphorylation | Uniprot | |
K116 | Ubiquitination | Uniprot | |
K122 | Ubiquitination | Uniprot | |
S132 | Phosphorylation | Uniprot | |
K138 | Ubiquitination | Uniprot | |
T164 | Phosphorylation | Uniprot | |
K176 | Ubiquitination | Uniprot | |
S181 | Phosphorylation | Uniprot | |
S185 | Phosphorylation | Uniprot | |
K190 | Ubiquitination | Uniprot | |
T192 | Phosphorylation | Uniprot | |
K197 | Ubiquitination | Uniprot | |
S200 | Phosphorylation | Uniprot |
研究背景
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. May be involved in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy.
Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Cell membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus. Recycling endosome membrane. Cell projection>Cilium. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Cilium basal body. Midbody. Cytoplasm. Cytoplasm>Cytoskeleton>Cilium axoneme.
Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803). In the GDP-bound form, present in the perinuclear region (PubMed:12221131). Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297). Localizes to tubular recycling endosome (PubMed:19864458). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). Non-phosphorylated RAB8A predominantly localizes to the cytoplasm whereas phosphorylated RAB8A localizes to the membrane (PubMed:26824392, PubMed:29125462, PubMed:30398148).
Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes (By similarity). Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling (By similarity). Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3, EHBP1 and EHBP1L1; at least in case of MICAL1, MICALCL, MICAL3 and EHBP1L1 two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts with EHD1. Interacts with MAP4K2 and SYTL4 (By similarity). Interacts with SGSM1 and SGSM3 (By similarity). Interacts with RABIF, RIMS2, RPH3A and RPH3A (By similarity). Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1 (By similarity). Interacts with DCDC1. Interacts with LRRK2; interaction facilitates phosphorylation of Thr-72. Interacts with RAB31P, GDI1, GDI2, CHM, CHML, RABGGTA, RABGGTB, TBC1D15 and INPP5B; these interactions are dependent on Thr-72 not being phosphorylated. Interacts with RILPL1 and RILPL2; these interactions are dependent on the phosphorylation of Thr-72 by LRRK2.
Belongs to the small GTPase superfamily. Rab family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Pancreatic secretion.
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