RGS20 Antibody - #DF9846
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9846, RRID:AB_2843040.
折りたたみ／展開
G(z)GAP; Gz selective GTPase activating protein; Gz-GAP; Gz-selective GTPase-activating protein; HGNC:14600; Regulator of G protein signaling 20; Regulator of G-protein signaling 20; Regulator of G-protein signaling Z1; Regulator of Gz selective protein signaling 1; Regulator of Gz-selective protein signaling 1; RGS20; RGS20_HUMAN; RGSZ1; ZGAP1;
免疫原
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
- O76081 RGS20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPQLSQDNQECLQKHFSRPSIWTQFLPLFRAQRYNTDIHQITENEGDLRAVPDIKSFPPAQLPDSPAAPKLFGLLSSPLSSLARFFSHLLRRPPPEAPRRRLDFSPLLPALPAARLSRGHEELPGRLSLLLGAALALPGRPSGGRPLRPPHPVAKPREEDATAGQSSPMPQMGSERMEMRKRQMPAAQDTPGAAPGQPGAGSRGSNACCFCWCCCCSCSCLTVRNQEDQRPTIASHELRADLPTWEESPAPTLEEVNAWAQSFDKLMVTPAGRNAFREFLRTEFSEENMLFWMACEELKKEANKNIIEEKARIIYEDYISILSPKEVSLDSRVREVINRNMVEPSQHIFDDAQLQIYTLMHRDSYPRFMNSAVYKDLLQSLSEKSIEA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O76081 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S77 | Phosphorylation | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot |
研究背景
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).
Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).
N- and O-glycosylated in synapsomal membranes.
Serine phosphorylated in synapsomal membranes.
Sumoylated with SUMO1 and SUMO2 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits (By similarity).
Membrane>Lipid-anchor. Nucleus. Cytoplasm.
Note: Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation.
Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
Forms a complex with G(alpha)z/i2 subunits and mu-opioid receptors; the formation of this complex results in mu-opioid receptor desensitization. Interacts with OPRM1 (By similarity).
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