RHG24 Antibody - #DF9858
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| 製品: | RHG24 Antibody |
| カタログ: | DF9858 |
| タンパク質の説明: | Rabbit polyclonal antibody to RHG24 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 84 kDa; 84kD(Calculated). |
| ユニプロット: | Q8N264 |
| RRID: | AB_2843052 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9858, RRID:AB_2843052.
折りたたみ／展開
ARHGAP24; DKFZP564B1162; Filamin A associated RhoGAP; FILGAP; FLJ33877; p73; p73RhoGAP; RAC1 and CDC4 specific GTPase activating protein of 72kDa; RCGAP72; Rho GTPase activating protein 24; Rho type GTPase activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY SAR 88;
免疫原
Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.
- Q8N264 RHG24_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEENNDSTENPQQGQGRQNAIKCGWLRKQGGFVKTWHTRWFVLKGDQLYYFKDEDETKPLGTIFLPGNKVSEHPCNEENPGKFLFEVVPGGDRDRMTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRDNKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N264 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T62 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T393 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S406 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S415 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| K421 | Acetylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| S437 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T452 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S464 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S574 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T575 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T576 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T577 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T624 | Phosphorylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| T728 | Phosphorylation | Uniprot |
研究背景
Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.
Phosphorylated by ROCK, leading to activate the RacGAP activity.
Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell junction>Focal adhesion. Cell projection.
Note: Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions.
Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.
Interacts with FLNA.
The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.
参考文献
Application: WB Species: Pig Sample: neural cells
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