Phospho-Bcr (Tyr177) Antibody - #AF3154

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製品: Phospho-Bcr (Tyr177) Antibody
カタログ: AF3154
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Bcr (Tyr177)
アプリケーション: WB IF/ICC
反応性: Human, Mouse
予測: Zebrafish, Chicken
分子量: 130,160kDa; 143kD(Calculated).
ユニプロット: P11274
RRID: AB_2834589

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Zebrafish(89%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
Phospho-Bcr (Tyr177) Antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177.
RRID:
AB_2834589
引用形式: Affinity Biosciences Cat# AF3154, RRID:AB_2834589.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ALL; bcr; BCR/ABL FUSION GENE, INCLUDED; BCR/FGFR1 chimera protein; BCR/FGFR1 FUSION GENE, INCLUDED; BCR/PDGFRA FUSION GENE, INCLUDED; BCR_HUMAN; BCR1; Breakpoint cluster region; Breakpoint cluster region protein; CML; D22S11; D22S662; FGFR1/BCR chimera protein; PHL; Renal carcinoma antigen NY-REN-26;

免疫原

免疫原:
Uniprot:

P11274(BCR_HUMAN) >>Visit HPA database.

遺伝子(ID):
BCR(613)
タンパク質の説明:
Bcr is a serine/threonine and GTPase-activating protein (GAP). breakpoint cluster region protein. GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them.
タンパク質配列:
MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILASEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRARSNKDAKDPTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQNFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDIQREKRANKGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRSFSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLNVIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTAEPNWNEEFEIELEGSQTLRILCYEKCYNKTKIPKEDGESTDRLMGKGQVQLDPQALQDRDWQRTVIAMNGIEVKLSVKFNSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGIYRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYPNFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKEAVNKMSLHNLATVFGPTLLRPSEKESKLPANPSQPITMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKRQSILFSTEV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
89
Chicken
89
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11274 基板として

Site PTM Type Enzyme
M1 Acetylation
S18 Phosphorylation
Y58 Phosphorylation
S88 Phosphorylation
S122 Phosphorylation
T130 Phosphorylation
S139 Phosphorylation
Y177 Phosphorylation P06241 (FYN) , A9UF07 (BCR/ABL fusion) , P07332 (FES) , P08631 (HCK) , P00519 (ABL1) , A0A173G4P4 (Abl fusion) , P07948 (LYN)
K194 Acetylation
S201 Phosphorylation
S202 Phosphorylation
S205 Phosphorylation
K213 Acetylation
K214 Ubiquitination
S215 Phosphorylation
S221 Phosphorylation
S222 Phosphorylation
S227 Phosphorylation
Y231 Phosphorylation
S235 Phosphorylation
S236 Phosphorylation
S239 Phosphorylation
C240 S-Nitrosylation
Y246 Phosphorylation P07332 (FES)
S267 Phosphorylation
Y276 Phosphorylation P07332 (FES)
Y279 Phosphorylation P07332 (FES)
S281 Phosphorylation
Y283 Phosphorylation A9UF07 (BCR/ABL fusion) , P07332 (FES)
S299 Phosphorylation
S301 Phosphorylation
T302 Phosphorylation
S303 Phosphorylation
T310 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
Y328 Phosphorylation A9UF07 (BCR/ABL fusion)
T329 Phosphorylation
S340 Phosphorylation
S351 Phosphorylation
S354 Phosphorylation
S356 Phosphorylation
T358 Phosphorylation
T359 Phosphorylation
Y360 Phosphorylation A9UF07 (BCR/ABL fusion) , A0A173G4P4 (Abl fusion)
S374 Phosphorylation
S377 Phosphorylation
T385 Phosphorylation
T410 Phosphorylation
S429 Phosphorylation
T432 Phosphorylation
Y436 Phosphorylation
Y455 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
S462 Phosphorylation
S463 Phosphorylation
S467 Phosphorylation
S468 Phosphorylation
S473 Phosphorylation
K486 Ubiquitination
S488 Phosphorylation
K494 Ubiquitination
Y513 Phosphorylation
S515 Phosphorylation
S540 Phosphorylation
K549 Ubiquitination
Y554 Phosphorylation
K558 Ubiquitination
Y561 Phosphorylation
S572 Phosphorylation
K583 Ubiquitination
S586 Phosphorylation
Y591 Phosphorylation
Y598 Phosphorylation
S626 Phosphorylation
T634 Phosphorylation
T635 Phosphorylation
K636 Ubiquitination
S638 Phosphorylation
T641 Phosphorylation
Y644 Phosphorylation
K645 Ubiquitination
T651 Phosphorylation
S653 Phosphorylation
T654 Phosphorylation
K662 Ubiquitination
T693 Phosphorylation
S698 Phosphorylation
Y751 Phosphorylation
Y756 Phosphorylation
K795 Ubiquitination
S843 Phosphorylation
Y844 Phosphorylation
T845 Phosphorylation
Y852 Phosphorylation
S871 Phosphorylation
S894 Phosphorylation
T898 Phosphorylation
S906 Phosphorylation
Y910 Phosphorylation
S919 Phosphorylation
T921 Phosphorylation
K1000 Ubiquitination
T1018 Phosphorylation
S1030 Phosphorylation
S1035 Phosphorylation
S1039 Phosphorylation
K1047 Ubiquitination
T1049 Phosphorylation
K1061 Ubiquitination
K1066 Ubiquitination
Y1069 Phosphorylation
Y1089 Phosphorylation
K1102 Ubiquitination
K1197 Ubiquitination
S1264 Phosphorylation

PTMs - P11274 酵素として

Substrate Site Source
P27348 (YWHAQ) S232 Uniprot
P55196 (AFDN) T909 Uniprot
P63104 (YWHAZ) T232 Uniprot
Q15366 (PCBP2) S173 Uniprot
Q15366 (PCBP2) S189 Uniprot
Q15366 (PCBP2) T213 Uniprot
Q15366 (PCBP2) S272 Uniprot

研究背景

機能:

Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein (GAP) domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The amino terminus contains an intrinsic kinase activity. Functions as an important negative regulator of neuronal RAC1 activity (By similarity). Regulates macrophage functions such as CSF1-directed motility and phagocytosis through the modulation of RAC1 activity. Plays a major role as a RHOA GEF in keratinocytes being involved in focal adhesion formation and keratinocyte differentiation.

PTMs:

Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.

細胞の位置付け:

Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Axon. Cell junction>Synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer. Interacts with PDZK1. May interact with CCPG1 (By similarity). Interacts with FES/FPS, ABL1, PIK3R1 and GRB2. Interacts with HCK. Interacts with SH2D5. Interacts with DLG4.

タンパク質ファミリー:

The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.

The DH domain is involved in interaction with CCPG1.

The amino terminus contains an intrinsic kinase activity. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form. The C-terminus is a Rho-GAP domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. The protein has a unique structure having two opposing regulatory activities toward small GTP-binding proteins.

研究領域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

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