RIOK1 Antibody - #DF9888
| 製品: | RIOK1 Antibody |
| カタログ: | DF9888 |
| タンパク質の説明: | Rabbit polyclonal antibody to RIOK1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 66 kDa; 66kD(Calculated). |
| ユニプロット: | Q9BRS2 |
| RRID: | AB_2843082 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9888, RRID:AB_2843082.
折りたたみ／展開
AD034; bA288G3.1; RIO kinase 1 (yeast); RIO kinase 1; RIOK1; RIOK1_HUMAN; Serine/threonine-protein kinase rio1;
免疫原
- Q9BRS2 RIOK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BRS2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| R95 | Methylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| K411 | Methylation | Uniprot | |
| K426 | Methylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| Y466 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot |
研究背景
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation.
Cytoplasm>Cytosol.
Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus). Interacts with WDR77. Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1. Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation.
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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