SLIT3 Antibody - #DF9909
(2)
(6)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
SLIT3 Antibody detects endogenous levels of total SLIT3.
RRID:
AB_2843103
引用形式: Affinity Biosciences Cat# DF9909, RRID:AB_2843103.
引用形式: Affinity Biosciences Cat# DF9909, RRID:AB_2843103.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
FLJ10764; KIAA0814; MEGF 5; MEGF5; Multiple EGF-like domains protein 5; Multiple epidermal growth factor like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL 2; SLIL2; SLIT 1; Slit 2; Slit 3; Slit homolog 3 (Drosophila); Slit homolog 3; Slit homolog 3 protein; Slit-3; SLIT1; Slit2; Slit3; SLIT3_HUMAN;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質配列:
- O75094 SLIT3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75094 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| K473 | Acetylation | Uniprot | |
| K561 | Acetylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| T609 | Phosphorylation | Uniprot | |
| S1173 | Phosphorylation | Uniprot | |
| T1295 | O-Glycosylation | Uniprot | |
| T1385 | Phosphorylation | Uniprot | |
| T1387 | Phosphorylation | Uniprot | |
| S1388 | Phosphorylation | Uniprot | |
| Y1389 | Phosphorylation | Uniprot |
研究背景
機能:
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
細胞の位置付け:
Secreted.
組織特異性:
Predominantly expressed in thyroid.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
参考文献
1). A novel epithelial-mesenchymal transition (EMT)-related gene signature of predictive value for the survival outcomes in lung adenocarcinoma. Frontiers in Oncology, 2022
(PubMed: 36185284)
[IF=4.7]
Application: WB Species: Human Sample: LUAD tumor
Figure 2 The identification 4 prognostic EMT-related genes comprising the E-signature. (A) The standard deviation distribution of all mRNAs. In the left panel, location corresponding to the red columns were standard deviation (SD) of genes with frequency greater than 500, where SD was used as the abscissa and the vertical axis represented the number of genes. The right panel showed that the gene frequency distribution chart obtained by LASSO analysis. The green baseline meant that frequency was equal to 500, the part exceeded the green line was genes with frequency greater than 500. (B) KM curves showed the overall survival (OS) of patients grouped according to expression patterns of 4 prognostic EMT-related genes comprising the E-signature in TCGA-LUAD datasets. (C) Differential expression analysis of DKK1, LOXL2, MGP and SLIT3 originated from TCGA-LUAD dataset. (D) The expression of 4 genes in fresh LUAD tumor samples (T) and adjacent normal-frozen tissues (N) detected by Western blot
2). Targeting Bioinformatics Predicted Biomarkers Associated with Cell Proliferation and Migration for Treating Gliomas: Preclinical Studies in a GL261 Mouse Model. Neuroglia, 2023
Application: IHC Species: Human Sample:
Figure 1. Expressions of PLXNB2, SLIT3, and SPON1 in human high-grade gliomas. Representative immunohistochemistry (IHC) images for PLXNB2 (A), SLIT3 (B), and SPON1 (C) in human GBM tumor sections. (D) Negative control in a low-grade glioma for SLIT3. Magnification is 20× for slides in (A–D). (E) Percentage (%) of patient protein expression (IHC) for PLXNB2, SLIT3, and SPON1 in high-grade (HG) gliomas highly expressed (HG E) or not expressed (HG NE). The % of patient expression for each biomarker in HG was 80% or higher.
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