CCL1 Antibody - #DF9910

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製品: CCL1 Antibody
カタログ: DF9910
タンパク質の説明: Rabbit polyclonal antibody to CCL1
アプリケーション: WB IHC
反応性: Human
分子量: 11 kDa; 11kD(Calculated).
ユニプロット: P22362
RRID: AB_2843104

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MS Report
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
CCL1 Antibody detects endogenous levels of total CCL1.
RRID:
AB_2843104
引用形式: Affinity Biosciences Cat# DF9910, RRID:AB_2843104.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C-C motif chemokine 1; Ccl1; CCL1_HUMAN; Chemokine CC Motif Ligand 1; inflammatory cytokine I-309; P500; SCYA1; SISe; small inducible cytokine A1; Small-inducible cytokine A1; T lymphocyte secreted protein I-309; T lymphocyte secreted protein I-309; T lymphocyte-secreted protein I-309; TCA3;

免疫原

免疫原:
Uniprot:

P22362(CCL1_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCL1(6346)
タンパク質配列:
MQIITTALVCLLLAGMWPEDVDSKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

研究背景

機能:

Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the intercrine beta (chemokine CC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). Iron Oxide Nanoparticles Engineered Macrophage-Derived Exosomes for Targeted Pathological Angiogenesis Therapy. ACS nano, 2024 (PubMed: 38412252) [IF=17.1]

Application: WB    Species: Mouse    Sample:

Figure 3 Ferroptosis-inducing and immuno-modulatory properties of ESIONPs@EXO. (A) The protein levels of NOX1, CD71, COX2, and GPX4 in ESIONPs@EXO-treated C166 determined by Western blot. (B) Statistical result of the protein levels in ESIONPs@EXO-treated C166. (C) Relative GSH level in ESIONPs@EXO-treated C166. (D) TEM representative images of mitochondria in C166 cells after treatment with ESIONPs@EXO for 24 h. Scale bar: 500 nm (upper) and 200 nm (lower). (E) Contents of various cytokines/chemokines in ESIONPs@EXO determined by protein array analysis. (F) The protein levels of CCL1, TIMP1, TIMP2, TNFα, IL-6, IL-9, CX3CL1, and CCL3 in EXO and ESIONPs@EXO determined by Western blot. (G) Statistical results of the protein levels in ESIONPs@EXO.
2). A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner. ANGIOGENESIS, 2021 (PubMed: 33484377) [IF=9.8]

Application: IF/ICC    Species: human    Sample: CSCC

Fig. 5 |Sp1high LECs are fundamental to LVEM formation and lymphatic metastasis.i Immunofuorescence staining was applied to analyse Sp1 (purple), CCL1 (red), CD163 (green) and DAPI (blue)expression in CSCC tissues (Scale bar, 50 μm).

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