製品: CXCL13 Antibody
カタログ: DF9918
タンパク質の説明: Rabbit polyclonal antibody to CXCL13
アプリケーション: WB
反応性: Human, Monkey
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 13 kDa; 13kD(Calculated).
ユニプロット: O43927
RRID: AB_2843112

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Monkey
予測:
Pig(86%), Bovine(86%), Sheep(86%), Rabbit(86%), Dog(86%)
クローナリティ:
Polyclonal
特異性:
CXCL13 Antibody detects endogenous levels of total CXCL13.
RRID:
AB_2843112
引用形式: Affinity Biosciences Cat# DF9918, RRID:AB_2843112.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ANGIE; ANGIE2; B cell attracting chemokine 1; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BCA-1; BLC; BLR1L; C-X-C motif chemokine 13; Chemokine (C-X-C motif) ligand 13; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; CXL13_HUMAN; SCYB13; Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13; Small-inducible cytokine B13;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O43927 CXL13_HUMAN:

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

タンパク質配列:
MKFISTSLLLMLLVSSLSPVQGVLEVYYTSLRCRCVQESSVFIPRRFIDRIQILPRGNGCPRKEIIVWKKNKSIVCVDPQAEWIQRMMEVLRKRSSSTLPVPVFKRKIP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Bovine
86
Sheep
86
Dog
86
Rabbit
86
Xenopus
57
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43927 基板として

Site PTM Type Enzyme
S39 Phosphorylation
S40 Phosphorylation
K63 Sumoylation
K69 Ubiquitination

研究背景

機能:

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

タンパク質ファミリー:

Belongs to the intercrine alpha (chemokine CxC) family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). Long Noncoding RNA SNHG5 Knockdown Alleviates Neuropathic Pain by Targeting the miR-154-5p/CXCL13 Axis. NEUROCHEMICAL RESEARCH, 2020 (PubMed: 32248399) [IF=3.7]

Application: WB    Species: mouse    Sample:

Fig. 5 |CXCL13 is a down-stream target of miR-154-5p. a The binding site of miR-154-5p on CXCL13 was shown. b The interaction between miR-154-5p and CXCL13 was verified using luciferase activity. c The CXCL13 protein level was detected at day 3, day 7 and day 14 after SNL model establishment.

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For Research Use Only.
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