MCM2 Antibody - #AF0206

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製品: MCM2 Antibody
カタログ: AF0206
タンパク質の説明: Rabbit polyclonal antibody to MCM2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Rabbit, Dog
分子量: 120kDa; 102kD(Calculated).
ユニプロット: P49736
RRID: AB_2833393

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(86%), Horse(86%), Rabbit(86%), Dog(86%)
クローナリティ:
Polyclonal
特異性:
MCM2 Antibody detects endogenous levels of total MCM2.
RRID:
AB_2833393
引用形式: Affinity Biosciences Cat# AF0206, RRID:AB_2833393.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050;

免疫原

免疫原:
Uniprot:

P49736(MCM2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MCM2(4171)
タンパク質の説明:
MCM2 a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
タンパク質配列:
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Horse
86
Dog
86
Rabbit
86
Bovine
69
Xenopus
67
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49736 基板として

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
S5 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
S7 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
T9 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)
T25 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7)
S31 Phosphorylation O00311 (CDC7)
S32 Phosphorylation
T35 Phosphorylation
T39 Phosphorylation
S40 Phosphorylation P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) , P24941 (CDK2)
S41 Phosphorylation O00311 (CDC7) , P24941 (CDK2) , P68400 (CSNK2A1) , P50613 (CDK7)
S53 Phosphorylation P68400 (CSNK2A1) , P24941 (CDK2) , P50613 (CDK7) , O00311 (CDC7)
T59 Phosphorylation P50613 (CDK7)
Y81 Phosphorylation
Y90 Phosphorylation
T106 Phosphorylation
S108 Phosphorylation P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , Q13535 (ATR) , O00311 (CDC7)
R126 Methylation
Y137 Phosphorylation
S139 Phosphorylation P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)
T158 Phosphorylation
S170 Phosphorylation
K178 Ubiquitination
K200 Ubiquitination
S209 Phosphorylation
K216 Acetylation
K216 Ubiquitination
S220 Phosphorylation O00311 (CDC7) , O14965 (AURKA)
K224 Ubiquitination
S229 Phosphorylation
Y234 Phosphorylation
R307 Methylation
C315 S-Nitrosylation
S381 Phosphorylation
K384 Ubiquitination
K394 Ubiquitination
K450 Ubiquitination
T457 Phosphorylation
K462 Ubiquitination
K469 Acetylation
K469 Ubiquitination
K476 Ubiquitination
S484 Phosphorylation
K492 Ubiquitination
T527 Phosphorylation
K529 Ubiquitination
K538 Ubiquitination
S566 Phosphorylation
K591 Ubiquitination
K613 Ubiquitination
R623 Methylation
R677 Methylation
K722 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K742 Acetylation
K742 Ubiquitination
K745 Ubiquitination
R751 Methylation
K752 Ubiquitination
S754 Phosphorylation
T757 Phosphorylation
T763 Phosphorylation
S769 Phosphorylation
S801 Phosphorylation
T857 Phosphorylation
K863 Ubiquitination
K868 Ubiquitination
S878 Phosphorylation
K896 Acetylation
K896 Ubiquitination

研究背景

機能:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.

PTMs:

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.

タンパク質ファミリー:

Belongs to the MCM family.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

参考文献

1). MiR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Molecular Therapy-Oncolytics, 2021 (PubMed: 34377766) [IF=5.7]

Application: WB    Species: Human    Sample: breast cancer cells

Figure 3 High expression of miR-301 can promote the invasion, proliferation of breast cancer cells as well as inhibit their apoptosis. A: Detection of miR-301 expression level by RT-qPCR; B:Detection of breast cancer cell proliferation by CCK-8; C: Distribution chart of breast cancer cell cycle; D: Detection of migration ability of breast cancer cells by Transwell; E, The relative number of invasive breast cancer cells, as measured by Transwell assay. F: Apoptosis rate of breast cancer cells; G: Detection of mRNA expression level of proliferation-related factor MCM2, migration- and invasion-related factors MMP2, apoptosis-related factors caspase-3 by RT-qPCR; H: Detection of protein expression level of proliferation-related factor MCM2, migration- and invasion-related factors MMP2,apoptosis-related factors caspase-3 by Western blot; The continuous variables were expressed by mean ± standard deviation; * P < 0.05 compared with NC group; Paired Sample t-test,Independent Sample t-test or one-way ANOVA were selected to test the inter-group differences of continuous variables; The difference of data of each group at different time was analyzed by Two-way ANOVA. We repeated the experiment three times in order to obtain accurate and stable results

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