TJP3 Antibody - #DF9961
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9961, RRID:AB_2843155.
折りたたみ／展開
Tight junction protein 3; Tight junction protein ZO-3; Tight junction protein ZO3; TJP3; ZO3; ZO3_HUMAN; Zona occludens protein 3; Zonula occludens protein 3;
免疫原
- O95049 ZO3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95049 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S27 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| Y130 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| R352 | Methylation | Uniprot | |
| R357 | Methylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| Y361 | Phosphorylation | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| Y479 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| R518 | Methylation | Uniprot | |
| S564 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| T679 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| S905 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot |
研究背景
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions. Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition. With TJP1 and TJP2, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).
Phosphorylated (By similarity).
Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Nucleus.
Note: Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23608536). Shows an epithelial-specific tight junction localization in a TJP1/TJP2-dependent fashion (By similarity).
Interacts with occludin OCLN, claudins and TPJ1 (By similarity). Interacts with PATJ (By similarity). Interacts with UBN1. Interacts with FASLG. Interacts with CCND1.
Belongs to the MAGUK family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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