Phospho-NCF1/p47-phox (Ser359) Antibody - #AF3167

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製品: Phospho-NCF1/p47-phox (Ser359) Antibody
カタログ: AF3167
タンパク質の説明: Rabbit polyclonal antibody to Phospho-NCF1/p47-phox (Ser359)
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
分子量: 45kDa; 45kD(Calculated).
ユニプロット: P14598
RRID: AB_2834599

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-NCF1/p47-phox (Ser359) Antibody detects endogenous levels of NCF1/p47-phox only when phosphorylated at Serine 359.
RRID:
AB_2834599
引用形式: Affinity Biosciences Cat# AF3167, RRID:AB_2834599.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; Nox-organizing protein 2; NOXO2; p47 phox; p47-phox; SH3 and PX domain containing protein 1A; SH3 and PX domain-containing protein 1A; SH3PXD1A;

免疫原

免疫原:
Uniprot:

P14598(NCF1_HUMAN) >>Visit HPA database.

遺伝子(ID):
NCF1(653361)
発現特異性:
P14598 NCF1_HUMAN:

Detected in peripheral blood monocytes and neutrophils (at protein level).

タンパク質の説明:
The 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase is found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains.
タンパク質配列:
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV

PTMs - P14598 基板として

Site PTM Type Enzyme
Y41 Phosphorylation
T45 Phosphorylation
Y48 Phosphorylation
T133 Phosphorylation Q9NWZ3 (IRAK4)
T138 Phosphorylation Q9NWZ3 (IRAK4)
Y139 Phosphorylation
T153 Phosphorylation Q9NWZ3 (IRAK4)
S208 Phosphorylation P68400 (CSNK2A1)
Y279 Phosphorylation
S283 Phosphorylation P68400 (CSNK2A1)
S288 Phosphorylation Q9NWZ3 (IRAK4)
S303 Phosphorylation P17252 (PRKCA) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB)
S304 Phosphorylation Q05513 (PRKCZ) , P05771-2 (PRKCB) , P17252 (PRKCA) , P31749 (AKT1) , Q05655 (PRKCD)
S310 Phosphorylation
S315 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA) , Q05513 (PRKCZ) , Q05655 (PRKCD)
S320 Phosphorylation Q9NWZ3 (IRAK4) , P17612 (PRKACA) , P05771-2 (PRKCB) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P17252 (PRKCA)
S328 Phosphorylation Q05655 (PRKCD) , P31749 (AKT1) , P17252 (PRKCA) , P17612 (PRKACA) , Q05513 (PRKCZ) , P05771 (PRKCB)
S345 Phosphorylation Q16539 (MAPK14) , Q9NWZ3 (IRAK4) , P28482 (MAPK1) , P27361 (MAPK3)
S348 Phosphorylation P68400 (CSNK2A1) , P28482 (MAPK1) , P27361 (MAPK3) , Q9NWZ3 (IRAK4) , Q16539 (MAPK14)
T356 Phosphorylation Q9NWZ3 (IRAK4)
S359 Phosphorylation Q9NWZ3 (IRAK4) , Q05513 (PRKCZ) , P05771-2 (PRKCB) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA)
S370 Phosphorylation Q05513 (PRKCZ) , Q9NWZ3 (IRAK4) , P05771 (PRKCB) , Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA)
S379 Phosphorylation Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , P17252 (PRKCA)
S381 Phosphorylation
T382 Phosphorylation

研究背景

機能:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

PTMs:

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

細胞の位置付け:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in peripheral blood monocytes and neutrophils (at protein level).

サブユニット構造:

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity).

タンパク質ファミリー:

The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission. ACS Nano, 2020 (PubMed: 32057235) [IF=17.1]

Application: WB    Species: rat    Sample: PC12

Figure S6 Oxidized rGO increased lipid peroxidation and NOX2 activation in PC12 cells. Oxidized rGO (c-rGO) was collected after pristine rGO (p-rGO) was cultured with PC12 cells for 3 h. (A-B) PC12 cells were pretreated with NAC for 30 min before being treated with two kinds of rGO (20 µg/mL) for 1 h and stained with BODIPY 581/591 C11 reagent. The lipid peroxidation degrees were quantified via FITC fluorescence intensity, with n = 10 fields per experimental condition from 3 independent tests. Scale bar: 100 μm. (C-D) After PC12 cells were treated as described in (A), plasma membrane proteins were isolated to detect the expression levels of NOX2, p-p47phox, p-p67phox and Rac2. The protein levels were quantified after normalization to ATPase. The results represent the mean ± SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001 compared with the control group and with the p-rGO group.
2). Preclinical efficacy of TZG in myofascial pain syndrome by impairing PI3K-RAC2 signaling-mediated neutrophil extracellular traps. iScience, 2023 (PubMed: 37860777) [IF=5.8]

Application: WB    Species: Rat    Sample:

Figure 5 TZG significantly regulated the expression of NETs formation-related indicators, and the corresponding proteins in PI3K-RAC2 signaling (A–E). Serum levels of dsDNA, IL-8, TNF-α, CitH3, and MPO detected by ELISA analyses, respectively. Samples in (A)∼(E) were obtained from the blood supernatant of rats in different groups. (F–I) The ratios of p-PI3K/PI3K and p-P47/P47, and the expression levels of RAC2 and PAD4 proteins detected by western blot analyses, respectively. Samples in (F)∼(I) were obtained from MTrPs tissues of rats in different groups. The number of rats in each group was 8. Data are expressed as the mean ± S.D. “∗,” “∗∗,” and “∗∗∗,” p < 0.05, p < 0.01, and p < 0.001, respectively, comparison with the normal control group. “#,” “##,” and “###,” p < 0.05, p < 0.01, and p < 0.001, respectively, comparison with the MPS model group.
3). CHANGES IN THE RAGE/NADPH OXIDASE SIGNALING PATHWAY AND OXIDATIVE STRESS LEVELS IN SH-SY5Y CELLS EXPOSED TO HIGH LEVELS OF FLUORIDE.. Fluoride, 2022 [IF=0.9]

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