UBQLN2 Antibody - #DF9980
| 製品: | UBQLN2 Antibody |
| カタログ: | DF9980 |
| タンパク質の説明: | Rabbit polyclonal antibody to UBQLN2 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 66 kDa; 66kD(Calculated). |
| ユニプロット: | Q9UHD9 |
| RRID: | AB_2843172 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF9980, RRID:AB_2843172.
折りたたみ／展開
bA431N15.1; CHAP 1; CHAP1; CHAP1/DSK2; Dsk 2; Dsk2; DSK2 homolog; hPLIC 2; hPLIC2; HRIHFB2157; LIC 2; LIC2; N4BP4; Nedd4 binding protein 4; PLIC 2; PLIC2; Protein linking IAP with cytoskeleton 2; RIHFB2157; Ubiquilin 2; Ubiquilin2; Ubiquitin like product Chap1/Dsk2; UBQLN 2;
免疫原
- Q9UHD9 UBQL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UHD9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| R249 | Methylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| R269 | Methylation | Uniprot |
研究背景
Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs).
Degraded during macroautophagy.
Cytoplasm. Nucleus. Membrane. Cytoplasmic vesicle>Autophagosome.
Note: Colocalizes with a subset of proteasomes, namely those that are cytoskeleton associated or free in the cytosol. Associated with fibers in mitotic cells.
Homodimer. Forms heterodimer with UBQLN1. Binds UBE3A and BTRC. Interacts with the 19S proteasome subunit. Interacts with C9orf72. Interacts with HNRNPA1 and HNRNPU. Found in a complex with UBQLN1 and MAP1LC3A/B/C. Interacts with EPS15, EPN1 and EPN2. Interacts with HERPUD1. Interacts with RAD23A. Interacts with TARDBP. Interacts (via C-terminus) with FAF2 (via N-terminus). Interacts with UBQLN4. Binds CD47 (By similarity).
The ubiquitin-like domain is essential for its inhibitory effect on GPCR endocytosis. Mediates its association with the subunits of the proteasome.
The UBA domain is essential for its association with microtubule-associated protein 1 light chain 3 (MAP1LC3). Mediates its association with ubiquitinated substrates.
Dimerization is dependent upon the central region of the protein containing the STI1 domains and is independent of its ubiquitin-like and UBA domains.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.