UBE4B Antibody - #DF9997

(1)  
製品: UBE4B Antibody
カタログ: DF9997
タンパク質の説明: Rabbit polyclonal antibody to UBE4B
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 146 kDa; 146kD(Calculated).
ユニプロット: O95155
RRID: AB_2843189

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(86%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
UBE4B Antibody detects endogenous levels of total UBE4B.
RRID:
AB_2843189
引用形式: Affinity Biosciences Cat# DF9997, RRID:AB_2843189.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; E4; HDNB1; Homozygously deleted in neuroblastoma 1; KIAA0684; mKIAA0684; RP23 173D8.1; Ube4b; UBE4B_HUMAN; Ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; UBOX3; UFD2; UFD2a; Ufd2p;

免疫原

免疫原:
Uniprot:

O95155(UBE4B_HUMAN) >>Visit HPA database.

遺伝子(ID):
UBE4B(10277)
発現特異性:
O95155 UBE4B_HUMAN:

Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788).

タンパク質配列:
MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTPSFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSSPSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Dog
92
Rabbit
92
Zebrafish
86
Chicken
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95155 基板として

Site PTM Type Enzyme
M1 Acetylation
R10 Methylation
T22 Phosphorylation
S23 Phosphorylation
T26 Phosphorylation
T27 Phosphorylation
T30 Phosphorylation
S31 Phosphorylation
T61 Phosphorylation
T64 Phosphorylation
S65 Phosphorylation
S76 Phosphorylation
S78 Phosphorylation
S79 Phosphorylation
S83 Phosphorylation
S84 Phosphorylation
S86 Phosphorylation
S87 Phosphorylation
S88 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
T95 Phosphorylation
S99 Phosphorylation
S101 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
C113 S-Nitrosylation
S116 Phosphorylation
S118 Phosphorylation
S124 Phosphorylation
S141 Phosphorylation
S143 Phosphorylation
K145 Ubiquitination
S149 Phosphorylation
S154 Phosphorylation
K192 Ubiquitination
S224 Phosphorylation
S238 Phosphorylation
S307 Phosphorylation
S352 Phosphorylation
S383 Phosphorylation
S392 Phosphorylation
K442 Ubiquitination
K542 Ubiquitination
K558 Ubiquitination
K575 Ubiquitination
K610 Ubiquitination
K702 Ubiquitination
T705 Phosphorylation
T709 Phosphorylation
Y710 Phosphorylation
K794 Ubiquitination
S798 Phosphorylation
K801 Sumoylation
K801 Ubiquitination
S803 Phosphorylation
T807 Phosphorylation
K827 Ubiquitination
Y929 Phosphorylation
K951 Ubiquitination
K964 Ubiquitination
S969 Phosphorylation
K972 Ubiquitination
K989 Ubiquitination
Y1026 Phosphorylation
T1034 Phosphorylation
K1056 Ubiquitination
K1058 Ubiquitination
K1106 Ubiquitination
K1132 Ubiquitination
K1143 Ubiquitination
K1150 Ubiquitination
K1169 Ubiquitination
K1180 Ubiquitination
K1189 Ubiquitination
K1196 Ubiquitination
K1203 Ubiquitination
K1205 Ubiquitination
K1210 Ubiquitination
K1217 Ubiquitination
S1249 Phosphorylation
S1256 Phosphorylation
S1265 Phosphorylation
T1267 Phosphorylation
T1274 Phosphorylation
S1278 Phosphorylation

研究背景

機能:

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.

PTMs:

Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in differentiated myotubes (at protein level). Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.

サブユニット構造:

Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B.

タンパク質ファミリー:

The U-box domain is required for the ubiquitin protein ligase activity.

Belongs to the ubiquitin conjugation factor E4 family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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