CaMKII beta/gamma Antibody - #AF9030
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9030, RRID:AB_2843221.
折りたたみ／展開
CaM kinase II; CaM kinase II gamma chain; CaM kinase II subunit gamma; CAMK; CAMK II; CaMK II subunit gamma; CAMK2G; CAMKG; CAMKII; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit alpha; CaM kinase II subunit delta; CAM2; CaMK-II subunit alpha; CaMK-II subunit delta; CAMK2A; CAMK2B; CAMKA; CAMKB; CAMKD; CaMKII alpha; KIAA0968;
免疫原
Expressed in skeletal muscle.
Q13554 KCC2B_HUMAN:Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
- Q13555 KCC2G_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATTATCTRFTDDYQLFEELGKGAFSVVRRCVKKTSTQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIHQILESVNHIHQHDIVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKGRVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ
- Q13554 KCC2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKAPRVPDILSSVRRGSGAPEAEGPLPCPSPAPFSPLPAPSPRISDILNSVRRGSGTPEAEGPLSAGPPPCLSPALLGPLSSPSPRISDILNSVRRGSGTPEAEGPSPVGPPPCPSPTIPGPLPTPSRKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13555/Q13554 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K57 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| T287 | Phosphorylation | Q13554 (CAMK2B) | Uniprot |
| K299 | Methylation | Uniprot | |
| K301 | Methylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| K342 | Acetylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| T400 | Phosphorylation | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y14 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| C273 | S-Nitrosylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Q13555 (CAMK2G) | Uniprot |
| T287 | Phosphorylation | Q13555 (CAMK2G) | Uniprot |
| K299 | Methylation | Uniprot | |
| K301 | Methylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Q13555 (CAMK2G) | Uniprot |
| S325 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| T382 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot |
PTMs - Q13555/Q13554 酵素として
| Substrate | Site | Source |
|---|---|---|
| O14490-2 (DLGAP1) | S349 | Uniprot |
| O14490-2 (DLGAP1) | S393 | Uniprot |
| P14921-1 (ETS1) | S251 | Uniprot |
| P14921-1 (ETS1) | S257 | Uniprot |
| P14921-1 (ETS1) | S282 | Uniprot |
| P14921-1 (ETS1) | S285 | Uniprot |
| P16949 (STMN1) | S16 | Uniprot |
| P26678 (PLN) | T17 | Uniprot |
| P35222 (CTNNB1) | T332 | Uniprot |
| P35222 (CTNNB1) | T472 | Uniprot |
| P35222 (CTNNB1) | S552 | Uniprot |
| P42224 (STAT1) | S727 | Uniprot |
| P49840 (GSK3A) | S21 | Uniprot |
| Q01970-1 (PLCB3) | S537 | Uniprot |
| Q12879-1 (GRIN2A) | S1291 | Uniprot |
| Q13131 (PRKAA1) | T183 | Uniprot |
| Q13224 (GRIN2B) | S383 | Uniprot |
| Q13224 (GRIN2B) | S1303 | Uniprot |
| Q13554-1 (CAMK2B) | T287 | Uniprot |
| Q9NQC7 (CYLD) | S362 | Uniprot |
| Q9NQC7 (CYLD) | S418 | Uniprot |
| Q9NQC7 (CYLD) | S772 | Uniprot |
| Substrate | Site | Source |
|---|---|---|
| O60266-1 (ADCY3) | S1076 | Uniprot |
| P00533-1 (EGFR) | S1070 | Uniprot |
| P00533-1 (EGFR) | S1071 | Uniprot |
| P07101-1 (TH) | S19 | Uniprot |
| P10276 (RARA) | T210 | Uniprot |
| P17600-2 (SYN1) | S568 | Uniprot |
| P17600-2 (SYN1) | S605 | Uniprot |
| P18846-1 (ATF1) | S63 | Uniprot |
| P21333-2 (FLNA) | S2515 | Uniprot |
| P21817-1 (RYR1) | S2843 | Uniprot |
| P28329 (CHAT) | T574 | Uniprot |
| P35270 (SPR) | S213 | Uniprot |
| P40763 (STAT3) | S727 | Uniprot |
| P42224-1 (STAT1) | S727 | Uniprot |
| P42261-1 (GRIA1) | S645 | Uniprot |
| P42261-1 (GRIA1) | S849 | Uniprot |
| P48058 (GRIA4) | S862 | Uniprot |
| P55042 (RRAD) | S273 | Uniprot |
| Q01970-1 (PLCB3) | S537 | Uniprot |
| Q03060-16 (CREM) | S87 | Uniprot |
| Q13555 (CAMK2G) | S280 | Uniprot |
| Q13555 (CAMK2G) | T287 | Uniprot |
| Q13555 (CAMK2G) | S319 | Uniprot |
| Q15121-1 (PEA15) | S116 | Uniprot |
| Q15746-1 (MYLK) | S1760 | Uniprot |
| Q92934 (BAD) | S134 | Uniprot |
| Q9UQL6 (HDAC5) | S259 | Uniprot |
| Q9UQL6 (HDAC5) | S498 | Uniprot |
研究背景
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization. It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed in skeletal muscle.
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.
The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse.
Note: In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other Ref.19). Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ. Interacts with FOXO3 (By similarity). Interacts (when in a kinase inactive state not associated with calmodulin) with ARC; leading to target ARC to inactive synapses (By similarity).
The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Amphetamine addiction.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Glioma. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Sensory system > Olfactory transduction.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
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