製品: Casein Kinase Igamma1 Antibody
カタログ: AF9033
タンパク質の説明: Rabbit polyclonal antibody to Casein Kinase Igamma1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 49kDa; 49kD(Calculated).
ユニプロット: Q9HCP0
RRID: AB_2843224

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(83%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
Casein Kinase Igamma1 Antibody detects endogenous levels of total Casein Kinase Igamma1.
RRID:
AB_2843224
引用形式: Affinity Biosciences Cat# AF9033, RRID:AB_2843224.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

9130020E21Rik; Casein kinase 1 gamma 1; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma-1; CKI gamma 1; CKI-gamma 1; CSNK1G1; EC 2.7.11.1; KC1G1_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MDHPSREKDERQRTTKPMAQRSAHCSRPSGSSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQRHK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Zebrafish
92
Pig
83
Xenopus
83
Chicken
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HCP0 基板として

Site PTM Type Enzyme
T14 Phosphorylation
T15 Phosphorylation
S22 Phosphorylation
S31 Phosphorylation
K49 Ubiquitination
K62 Ubiquitination
Y65 Phosphorylation
T66 Phosphorylation
Y69 Phosphorylation
Y88 Phosphorylation
Y91 Phosphorylation
S96 Phosphorylation
K166 Ubiquitination
Y194 Phosphorylation
K255 Ubiquitination
T258 Phosphorylation
K265 Ubiquitination
T309 Phosphorylation
K317 Ubiquitination
S344 Phosphorylation
S361 Phosphorylation
K411 Ubiquitination

PTMs - Q9HCP0 酵素として

Substrate Site Source
O75581 (LRP6) T1479 Uniprot
P25054 (APC) S1504 Uniprot
P25054 (APC) S1505 Uniprot
P25054 (APC) S1507 Uniprot
P25054 (APC) S1510 Uniprot
Q04206 (RELA) S536 Uniprot
Q12778 (FOXO1) S322 Uniprot

研究背景

機能:

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.

PTMs:

Autophosphorylated.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

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