CTNNA1/CTNNA2 Antibody - #AF9036

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製品: CTNNA1/CTNNA2 Antibody
カタログ: AF9036
タンパク質の説明: Rabbit polyclonal antibody to CTNNA1/CTNNA2
アプリケーション: WB IF/ICC
反応性: Human
分子量: 100kDa; 100kD,105kD(Calculated).
ユニプロット: P35221 | P26232
RRID: AB_2843227

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
CTNNA1/CTNNA2 Antibody detects endogenous levels of total CTNNA1/CTNNA2.
RRID:
AB_2843227
引用形式: Affinity Biosciences Cat# AF9036, RRID:AB_2843227.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha E-catenin; Cadherin associated protein 102kDa; Cadherin associated protein; Cadherin-associated protein; CAP 102; CAP102; Catenin (cadherin associated protein) alpha 1 102kDa; Catenin (cadherin associated protein), alpha 1, 102kDa; Catenin alpha 1; Catenin alpha-1; CTNA1_HUMAN; CTNNA 1; Ctnna1; FLJ36832; FLJ52416; MDPT2; NY REN 13 antigen; OTTHUMP00000224141; OTTHUMP00000224147; Renal carcinoma antigen NY REN 13; Renal carcinoma antigen NY-REN-13; Alpha catenin related protein; Alpha N catenin; Alpha N-catenin; Alpha-catenin-related protein; Cadherin associated protein related; Cancer/testis antigen 114; CAP R; CAPR; Catenin (cadherin associated protein) alpha 2; Catenin alpha 2; Catenin alpha-2; CT114; CTNA2_HUMAN; CTNN A2; CTNNA 2; CTNNA2; CTNR; DKFZp686H02198;

免疫原

免疫原:

A synthesized peptide derived from human CTNNA1/CTNNA2, corresponding to a region within C-terminal amino acids.

Uniprot:

P35221(CTNA1_HUMAN) >>Visit HPA database.

P26232(CTNA2_HUMAN) >>Visit HPA database.

遺伝子(ID):
CTNNA1(1495) | CTNNA2(1496)
発現特異性:
P35221 CTNA1_HUMAN:

Expressed ubiquitously in normal tissues.

P26232 CTNA2_HUMAN:

Expressed in neural tissues, with strongest expression in fetal and adult brain. Expressed in the developing cortical plate and marginal zone of 20-week-old human fetal brain.

タンパク質配列:
MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQATENFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI

MTSATSPIILKWDPKSLEIRTLTVERLLEPLVTQVTTLVNTSNKGPSGKKKGRSKKAHVLAASVEQATQNFLEKGEQIAKESQDLKEELVAAVEDVRKQGETMRIASSEFADDPCSSVKRGTMVRAARALLSAVTRLLILADMADVMRLLSHLKIVEEALEAVKNATNEQDLANRFKEFGKEMVKLNYVAARRQQELKDPHCRDEMAAARGALKKNATMLYTASQAFLRHPDVAATRANRDYVFKQVQEAIAGISNAAQATSPTDEAKGHTGIGELAAALNEFDNKIILDPMTFSEARFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMNNTGRKEKGDPLNIAIDKMTKKTRDLRRQLRKAVMDHISDSFLETNVPLLVLIEAAKSGNEKEVKEYAQVFREHANKLVEVANLACSISNNEEGVKLVRMAATQIDSLCPQVINAALTLAARPQSKVAQDNMDVFKDQWEKQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVIALQEGDVDTLDRTAGAIRGRAARVIHIINAEMENYEAGVYTEKVLEATKLLSETVMPRFAEQVEVAIEALSANVPQPFEENEFIDASRLVYDGVRDIRKAVLMIRTPEELEDDSDFEQEDYDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEEKAKIAEQVEIFHQEKSKLDAEVAKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVINAAKKIAEAGSRMDKLARAVADQCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELIVSGTGVQSTFTTFYEVDCDVIDGGRASQLSTHLPTCAEGAPIGSGSSDSSMLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYGTAAVNSPVVSWKMKAPEKKPLVKREKPEEFQTRVRRGSQKKHISPVQALSEFKAMDSF

PTMs - P35221/P26232 基板として

Site PTM Type Enzyme
T2 Phosphorylation
K12 Ubiquitination
K16 Ubiquitination
T38 Phosphorylation
T42 Phosphorylation
S44 Phosphorylation
K45 Ubiquitination
K50 Ubiquitination
K57 Ubiquitination
K75 Ubiquitination
K81 Ubiquitination
S83 Phosphorylation
K87 Ubiquitination
S118 Phosphorylation
Y148 Phosphorylation
K163 Ubiquitination
R165 Methylation
Y177 Phosphorylation
K178 Ubiquitination
K181 Ubiquitination
K186 Acetylation
K186 Ubiquitination
K193 Acetylation
K193 Ubiquitination
K199 Ubiquitination
K216 Ubiquitination
Y222 Phosphorylation
T223 Phosphorylation
Y237 Phosphorylation
K238 Ubiquitination
Y245 Phosphorylation
K246 Ubiquitination
S264 Phosphorylation
S268 Phosphorylation
Y280 Phosphorylation
S295 Phosphorylation
S297 Phosphorylation
S304 Phosphorylation
S311 Phosphorylation
S323 Phosphorylation
S361 Phosphorylation
S366 Phosphorylation
K370 Ubiquitination
T372 Phosphorylation
K384 Ubiquitination
S393 Phosphorylation
K414 Ubiquitination
K417 Ubiquitination
Y419 Phosphorylation
K429 Ubiquitination
S439 Phosphorylation
K448 Ubiquitination
S453 Phosphorylation
S455 Phosphorylation
S477 Phosphorylation
K478 Ubiquitination
K493 Ubiquitination
T506 Phosphorylation
S507 Phosphorylation
K533 Ubiquitination
T541 Phosphorylation
Y563 Phosphorylation
K577 Ubiquitination
Y619 Phosphorylation
T634 Phosphorylation
S641 Phosphorylation P68400 (CSNK2A1)
T645 Phosphorylation
S652 Phosphorylation
T654 Phosphorylation
S655 Phosphorylation
T658 Phosphorylation
S668 Phosphorylation
K681 Ubiquitination
K683 Ubiquitination
S690 Phosphorylation
K695 Ubiquitination
S696 Phosphorylation
K737 Ubiquitination
S744 Phosphorylation
K747 Acetylation
K747 Ubiquitination
S754 Phosphorylation
K758 Ubiquitination
C767 S-Nitrosylation
S770 Phosphorylation
C772 S-Nitrosylation
K773 Ubiquitination
Y779 Phosphorylation
K797 Ubiquitination
Y837 Phosphorylation
K842 Acetylation
K842 Ubiquitination
S846 Phosphorylation
S851 Phosphorylation
S858 Phosphorylation
S886 Phosphorylation
S898 Phosphorylation
K901 Methylation
K901 Ubiquitination
S905 Phosphorylation
Site PTM Type Enzyme
K15 Ubiquitination
T41 Phosphorylation
S42 Phosphorylation
S151 Phosphorylation
S302 Phosphorylation
S309 Phosphorylation
S321 Phosphorylation
K415 Ubiquitination
Y417 Phosphorylation
T535 Phosphorylation
R621 Methylation
S640 Phosphorylation
S651 Phosphorylation
T653 Phosphorylation
S654 Phosphorylation
T657 Phosphorylation
S667 Phosphorylation
K696 Ubiquitination
T738 Phosphorylation
S739 Phosphorylation
S753 Phosphorylation
Y778 Phosphorylation
K889 Acetylation
K889 Ubiquitination
S939 Phosphorylation
S945 Phosphorylation

研究背景

機能:

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

PTMs:

Sumoylated.

Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction.
Note: Found at cell-cell boundaries and probably at cell-matrix boundaries.

Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed ubiquitously in normal tissues.

サブユニット構造:

Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds AFDN and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1 (By similarity). Interacts with vinculin/VCL. Component of an cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1.

タンパク質ファミリー:

Belongs to the vinculin/alpha-catenin family.

機能:

May function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system (By similarity). Required for proper regulation of cortical neuronal migration and neurite growth. It acts as negative regulator of Arp2/3 complex activity and Arp2/3-mediated actin polymerization. It thereby suppresses excessive actin branching which would impair neurite growth and stability. Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation (By similarity).

細胞の位置付け:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm. Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell projection>Axon. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in neural tissues, with strongest expression in fetal and adult brain. Expressed in the developing cortical plate and marginal zone of 20-week-old human fetal brain.

サブユニット構造:

Interacts with CDH1 and CDH2 (By similarity). Interacts with ZNF639; recruits CTNNA2 to the nucleus. Interacts with F-actin.

タンパク質ファミリー:

Belongs to the vinculin/alpha-catenin family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). Microcystin-LR accelerates follicular atresia in mice via JNK-mediated adherent junction damage of ovarian granulosa cells. Ecotoxicology and Environmental Safety, 2023 (PubMed: 36731181) [IF=6.8]

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