eRF3/GSPT1 Antibody - #AF9062
| 製品: | eRF3/GSPT1 Antibody |
| カタログ: | AF9062 |
| タンパク質の説明: | Rabbit polyclonal antibody to eRF3/GSPT1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 55kDa; 56kD(Calculated). |
| ユニプロット: | P15170 |
| RRID: | AB_2843253 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9062, RRID:AB_2843253.
折りたたみ／展開
551G9.2; Anti Eukaryotic Release Factor 3a; Anti G1 to S phase transition 1; Anti GST1, homolog of yeast; eRF 3a; eRF3a; G1 to S phase transition 1; G1 to S phase transition protein 1 homolog; G1 to S phase transition protein; GSPT 1; GSPT1; GST 1; GST1; GTP binding protein GST1 HS; Transition protein 1;
免疫原
- P15170 ERF3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P15170 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Methylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K208 | Acetylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| C453 | S-Nitrosylation | Uniprot | |
| K469 | Acetylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K490 | Acetylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K493 | Acetylation | Uniprot | |
| K493 | Ubiquitination | Uniprot |
研究背景
Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.
Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. The ETF1-GSPT1 complex interacts with JMJD4. Interacts with PABPC1 (By similarity). Interacts with SHFL.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.
研究領域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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