Glucosidase II alpha Antibody - #AF9072
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9072, RRID:AB_2843263.
折りたたみ／展開
Alpha glucosidase II alpha subunit; Alpha-glucosidase 2; G2AN; GANAB; GANAB_HUMAN; Glu II; Glucosidase alpha neutral AB; Glucosidase II alpha; Glucosidase II subunit alpha; GluII; KIAA0088; Neutral alpha glucosidase AB; Neutral alpha glucosidase AB precursor; Neutral alpha-glucosidase AB;
免疫原
Detected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).
- Q14697 GANAB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14697 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S36 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| C47 | S-Nitrosylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Methylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| N97 | N-Glycosylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| R164 | Methylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| R182 | Methylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T204 | Phosphorylation | Uniprot | |
| R206 | Methylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| K436 | Acetylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K472 | Sumoylation | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| R484 | Methylation | Uniprot | |
| Y489 | Phosphorylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| Y563 | Phosphorylation | Uniprot | |
| Y578 | Phosphorylation | Uniprot | |
| R590 | Methylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| R698 | Methylation | Uniprot | |
| T790 | Phosphorylation | Uniprot | |
| Y792 | Phosphorylation | Uniprot | |
| T796 | Phosphorylation | Uniprot | |
| T808 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| R863 | Methylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| K908 | Ubiquitination | Uniprot | |
| K930 | Ubiquitination | Uniprot | |
| S937 | Phosphorylation | Uniprot | |
| R944 | Methylation | Uniprot |
研究背景
Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.
Endoplasmic reticulum. Golgi apparatus. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Detected in placenta. Isoform 1 and isoform 2 are expressed in the kidney and liver.
Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).
Belongs to the glycosyl hydrolase 31 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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