製品: LHR Antibody
カタログ: AF9104
タンパク質の説明: Rabbit polyclonal antibody to LHR
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
予測: Dog
分子量: 80kDa; 79kD(Calculated).
ユニプロット: P22888
RRID: AB_2843294

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Dog(89%)
クローナリティ:
Polyclonal
特異性:
LHR Antibody detects endogenous levels of total LHR.
RRID:
AB_2843294
引用形式: Affinity Biosciences Cat# AF9104, RRID:AB_2843294.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor; Lutropin choriogonadotropic receptor; Lutropin-choriogonadotropic hormone receptor; ULG5;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P22888 LSHR_HUMAN:

Gonadal and thyroid cells.

タンパク質配列:
MKQRFSALQLLKLLLLLQPPLPRALREALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLINILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHAIDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGGWLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIYFAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVLFYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGSNKPSQSTLKLSTLHCQGTALLDKTRYTEC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
89
Pig
78
Bovine
78
Sheep
78
Chicken
78
Rabbit
78
Xenopus
71
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22888 基板として

Site PTM Type Enzyme
S494 Phosphorylation
S681 Phosphorylation
T688 Phosphorylation

研究背景

機能:

Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

PTMs:

Sulfated.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Gonadal and thyroid cells.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

参考文献

1). Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep. Animal Reproduction Science, 2023 (PubMed: 37541020) [IF=2.2]

2). miR-17-5p affects porcine granulosa cell growth and estradiol synthesis by targeting E2F1 gene. REPRODUCTION IN DOMESTIC ANIMALS, 2019 (PubMed: 31424586) [IF=1.7]

Application: WB    Species: porcine    Sample: porcine GCs

FIGURE 3 |miR‐17‐5p up‐regulates LHR, CYP19A1 and AREG expression and oestradiol synthesis in pGCs. All the experiments were done in porcine GCs 48 hr after transfected. (a) The mRNA expression levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by qRT‐PCR. (b) Protein levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by Western blot.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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