NIPA Antibody - #AF9132
| 製品: | NIPA Antibody |
| カタログ: | AF9132 |
| タンパク質の説明: | Rabbit polyclonal antibody to NIPA |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 55kDa; 55kD(Calculated). |
| ユニプロット: | Q86WB0 |
| RRID: | AB_2843322 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9132, RRID:AB_2843322.
折りたたみ／展開
hNIPA; NIPA_HUMAN; Nuclear interacting partner of ALK; Nuclear interacting partner of anaplastic lymphoma kinase; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; zc3hc1; Zinc finger C3HC type containing 1; Zinc finger C3HC-type protein 1;
免疫原
Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
- Q86WB0 NIPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPCEGQAFAVGVEKNWGAVVRSPEGTPQKIRQLIDEGIAPEEGGVDAKDTSATSQSVNGSPQAEQPSLESTSKEAFFSRVETFSSLKWAGKPFELSPLVCAKYGWVTVECDMLKCSSCQAFLCASLQPAFDFDRYKQRCAELKKALCTAHEKFCFWPDSPSPDRFGMLPLDEPAILVSEFLDRFQSLCHLDLQLPSLRPEDLKTMCLTEDKISLLLHLLEDELDHRTDERKTTIKLGSDIQVHVTACILSVCGWACSSSLESMQLSLITCSQCMRKVGLWGFQQIESSMTDLDASFGLTSSPIPGLEGRPERLPLVPESPRRMMTRSQDATFSPGSEQAEKSPGPIVSRTRSWDSSSPVDRPEPEAASPTTRTRPVTRSMGTGDTPGLEVPSSPLRKAKRARLCSSSSSDTSSRSFFDPTSQHRDWCPWVNITLGKESRENGGTEPDASAPAEPGWKAVLTILLAHKQSSQPAETDSMSLSEKSRKVFRIFRQWESLCSC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86WB0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| T52 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| T84 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S87 | Phosphorylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| Y137 | Phosphorylation | Q9UM73 (ALK) | Uniprot |
| K146 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S357 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| R363 | Methylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| T384 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S395 | Phosphorylation | P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| S407 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| T413 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot |
研究背景
Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dependent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.
Nucleus.
Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.
Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-354. Interacts with CCNB1.
The F-box-like region is required for the interaction with SKP1.
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