PDE4B/C/D Antibody - #AF9151
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9151, RRID:AB_2843341.
折りたたみ／展開
5''-cyclic phosphodiesterase 4B; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4B; cAMP-specific phosphodiesterase-4 B isoform; DKFZp686F2182; DPDE4; dunce-like phosphodiesterase E4; MGC126529; OTTHUMP00000010652; OTTHUMP00000010653; OTTHUMP00000010654; OTTHUMP00000010656; OTTHUMP00000232365; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B_HUMAN; PDE4B5; PDEIVB; Phosphodiesterase 4B; Phosphodiesterase 4B cAMP specific; Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila); 5''-cyclic phosphodiesterase 4C; cAMP specific 3' 5' cyclic phosphodiesterase 4C; cAMP-specific 3''; DPDE 1; DPDE1; MGC126222; PDE 21; PDE 4C; PDE21; Pde4c; PDE4C_HUMAN; Phosphodiesterase 4C; Phosphodiesterase 4C cAMP specific; Phosphodiesterase 4C, cAMP specific (phosphodiesterase E1 dunce homolog, Drosophila); Phosphodiesterase E1 dunce homolog; Phosphodiesterase E1 dunce homolog Drosophila; Phosphodiesterase type 4C; cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); STRK1;
免疫原
Expressed in brain, heart, lung and skeletal muscle.
Q08493 PDE4C_HUMAN:Expressed in various tissues but not in cells of the immune system.
Q08499 PDE4D_HUMAN:Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
- Q07343 PDE4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLWRGRRCCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLTTLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSAGLVLHATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQVLASLRSVRNNFTILTNLHGTSNKRSPAASQPPVSRVNPQEESYQKLAMETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLKTFRISSDTFITYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFMNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDEQNRDCQGLMEKFQFELTLDEEDSEGPEKEGEGHSYFSSTKTLCVIDPENRDSLGETDIDIATEDKSPVDT
- Q08493 PDE4C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENLGVGEGAEACSRLSRSRGRHSMTRAPKHLWRQPRRPIRIQQRFYSDPDKSAGCRERDLSPRPELRKSRLSWPVSSCRRFDLENGLSCGRRALDPQSSPGLGRIMQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQCLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELTHLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQEEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVAYHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASVLENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVLLLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKSQVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAEEEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT
- Q08499 PDE4D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT
PTMs - Q07343/Q08493/Q08499 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y47 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K238 | Methylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T646 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K309 | Methylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K387 | Sumoylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S746 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S54 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S138 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K251 | Methylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| K521 | Acetylation | Uniprot | |
| K536 | Acetylation | Uniprot | |
| K537 | Acetylation | Uniprot | |
| Y549 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | P28482 (MAPK1) | Uniprot |
研究背景
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Expressed in brain, heart, lung and skeletal muscle.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Cell projection>Cilium.
Expressed in various tissues but not in cells of the immune system.
Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
Apical cell membrane. Cytoplasm. Membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells.
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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