Phospho-GATA1 (Ser310) Antibody - #AF3185
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3185, RRID:AB_2834617.
折りたたみ／展開
Anemia, X-linked, without thrombocytopenia, included; ERYF 1; Eryf1; Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1 (globin transcription factor 1); GATA binding protein 1; GATA-1; GATA-binding factor 1; GATA1; GATA1_HUMAN; GF 1; GF-1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NF-E1 DNA-binding protein; NFE 1; NFE1; Nuclear factor erythroid 1; Transcription factor GATA1; XLANP; XLTDA; XLTT;
免疫原
- P15976 GATA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSSLGGTGAAEGPAGGFMVVAGGSGSGNCGEVASGLTLGPPGTAHLYQGLGPVVLSGPVSHLMPFPGPLLGSPTGSFPTGPMPPTTSTTVVAPLSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P15976 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S72 | Phosphorylation | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| K137 | Sumoylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Q9P286 (PAK5) | Uniprot |
| S178 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Q9P286 (PAK5) | Uniprot |
| Y223 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K233 | Acetylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K246 | Acetylation | Uniprot | |
| Y285 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K312 | Acetylation | Uniprot | |
| K314 | Acetylation | Uniprot | |
| K315 | Acetylation | Uniprot | |
| K316 | Acetylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).
Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Nucleus.
Erythrocytes.
May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo (By similarity). Interacts with EP300. Interacts with MED1, CCAR1 and CALCOCO1. Interacts with CEBPE.
The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding (By similarity).
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