PNPase Antibody - #AF9164
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9164, RRID:AB_2843354.
折りたたみ／展開
3' 5' RNA exonuclease; COXPD13; DFNB70; old-35; OLD35; PNPASE; PNPase 1; Polynucleotide phosphorylase like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial;
免疫原
- Q8TCS8 PNPT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAACRYCCSCLRLRPLSDGPFLLPRRDRALTQLQVRALWSSAGSRAVAVDLGNRKLEISSGKLARFADGSAVVQSGDTAVMVTAVSKTKPSPSQFMPLVVDYRQKAAAAGRIPTNYLRREIGTSDKEILTSRIIDRSIRPLFPAGYFYDTQVLCNLLAVDGVNEPDVLAINGASVALSLSDIPWNGPVGAVRIGIIDGEYVVNPTRKEMSSSTLNLVVAGAPKSQIVMLEASAENILQQDFCHAIKVGVKYTQQIIQGIQQLVKETGVTKRTPQKLFTPSPEIVKYTHKLAMERLYAVFTDYEHDKVSRDEAVNKIRLDTEEQLKEKFPEADPYEIIESFNVVAKEVFRSIVLNEYKRCDGRDLTSLRNVSCEVDMFKTLHGSALFQRGQTQVLCTVTFDSLESGIKSDQVITAINGIKDKNFMLHYEFPPYATNEIGKVTGLNRRELGHGALAEKALYPVIPRDFPFTIRVTSEVLESNGSSSMASACGGSLALMDSGVPISSAVAGVAIGLVTKTDPEKGEIEDYRLLTDILGIEDYNGDMDFKIAGTNKGITALQADIKLPGIPIKIVMEAIQQASVAKKEILQIMNKTISKPRASRKENGPVVETVQVPLSKRAKFVGPGGYNLKKLQAETGVTISQVDEETFSVFAPTPSAMHEARDFITEICKDDQEQQLEFGAVYTATITEIRDTGVMVKLYPNMTAVLLHNTQLDQRKIKHPTALGLEVGQEIQVKYFGRDPADGRMRLSRKVLQSPATTVVRTLNDRSSIVMGEPISQSSSNSQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TCS8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S59 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K289 | Acetylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| K306 | Acetylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| Y334 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| Y356 | Phosphorylation | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K419 | Ubiquitination | Uniprot | |
| K439 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| K521 | Acetylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K569 | Methylation | Uniprot | |
| K591 | Acetylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| T592 | Phosphorylation | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| Y626 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| T687 | Phosphorylation | Uniprot | |
| T721 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| T758 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S782 | Phosphorylation | Uniprot |
研究背景
RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.
Cytoplasm. Mitochondrion matrix. Mitochondrion intermembrane space>Peripheral membrane protein.
Homotrimer; in free form. Homooligomer. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. As part of the mitochondrial degradosome complex, interacts with GRSF1 in an RNA-dependent manner; the interaction enhances the activity of the complex. Interacts with TCL1A; the interaction has no effect on PNPT1 exonuclease activity.
Belongs to the polyribonucleotide nucleotidyltransferase family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
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