POLR3B Antibody - #AF9165
| 製品: | POLR3B Antibody |
| カタログ: | AF9165 |
| タンパク質の説明: | Rabbit polyclonal antibody to POLR3B |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Rat |
| 予測: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 128kDa; 128kD(Calculated). |
| ユニプロット: | Q9NW08 |
| RRID: | AB_2843355 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9165, RRID:AB_2843355.
折りたたみ／展開
C128; DNA directed RNA polymerase III 127.6 kDa polypeptide; DNA directed RNA polymerase III subunit B; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; DNA-directed RNA polymerase III subunit RPC2; FLJ10388; polr3b; Polymerase (RNA) III (DNA directed) polypeptide B; RNA polymerase III subunit C2; RNA polymerase III subunit RPC2; RPC2; RPC2_HUMAN;
免疫原
- Q9NW08 RPC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NW08 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T12 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| T707 | Phosphorylation | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| Y714 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | Uniprot | |
| K723 | Ubiquitination | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| K820 | Ubiquitination | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| K852 | Ubiquitination | Uniprot | |
| K859 | Methylation | Uniprot | |
| K859 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| S873 | Phosphorylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K1013 | Methylation | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| T1036 | Phosphorylation | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
Belongs to the RNA polymerase beta chain family.
研究領域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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