POLR3E Antibody - #AF9166
| 製品: | POLR3E Antibody |
| カタログ: | AF9166 |
| タンパク質の説明: | Rabbit polyclonal antibody to POLR3E |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 80kDa; 80kD(Calculated). |
| ユニプロット: | Q9NVU0 |
| RRID: | AB_2843356 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9166, RRID:AB_2843356.
折りたたみ／展開
DNA directed RNA polymerase III 80 kDa polypeptide; DNA-directed RNA polymerase III subunit RPC5; FLJ10509; KIAA1452; polymerase (RNA) III (DNA directed) polypeptide E (80kD); Polymerase (RNA) III (DNA directed) polypeptide E; RNA polymerase III 80 kDa subunit RPC5; RNA polymerase III subunit C5; RPC5; SIN;
免疫原
- Q9NVU0 RPC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANEEDDPVVQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPHLSAKIKPKQQKVELEMAIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTFCSSQTTSNTSRYAAALYRQGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGDSSQDEAEDDVKQITVRFSRPESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGLRDSRSEHERQYLLCPGSSGVENTELVKSPSEYLMMLMPPSQEEEKDKPVAPSNVLSMAQLRTLPLADQIKILMKNVKVMPFANLMSLLGPSIDSVAVLRGIQKVAMLVQGNWVVKSDILYPKDSSSPHSGVPAEVLCRGRDFVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHMAVVRINKGWEFILPYDGEFIKKHPDVVQRQHMLWTGIQAKLEKVYNLVKETMPKKPDAQSGPAGLVCGDQRIQVAKTKAQQNHALLERELQRRKEQLRVPAVPPGVRIKEEPVSEEGEEDEEQEAEEEPMDTSPSGLHSKLANGLPLGRAAGTDSFNGHPPQGCASTPVARELKAFVEATFQRQFVLTLSELKRLFNLHLASLPPGHTLFSGISDRMLQDTVLAAGCKQILVPFPPQTAASPDEQKVFALWESGDMSDQHRQVLLEIFSKNYRVRRNMIQSRLTQECGEDLSKQEVDKVLKDCCVSYGGMWYLKGTVQS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NVU0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S36 | Phosphorylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K465 | Methylation | Uniprot | |
| K467 | Methylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K498 | Sumoylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| K659 | Sumoylation | Uniprot | |
| K682 | Ubiquitination | Uniprot |
研究背景
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4.
研究領域
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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