Rab11-FIP4 Antibody - #AF9175
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9175, RRID:AB_2843365.
折りたたみ／展開
Rab11 family interacting protein 4; RAB11 FIP4; Rab11 FIP4 like; RGD1305063; RP23-271B20.4;
免疫原
Present at high level in testis (at protein level). Weakly expressed in other tissues.
- Q86YS3 RFIP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGGAGWSGAPAALLRSVRRLREVFEVCGRDPDGFLRVERVAALGLRFGQGEEVEKLVKYLDPNDLGRINFKDFCRGVFAMKGCEELLKDVLSVESAGTLPCAPEIPDCVEQGSEVTGPTFADGELIPREPGFFPEDEEEAMTLAPPEGPQELYTDSPMESTQSLEGSVGSPAEKDGGLGGLFLPEDKSLVHTPSMTTSDLSTHSTTSLISNEEQFEDYGEGDDVDCAPSSPCPDDETRTNVYSDLGSSVSSSAGQTPRKMRHVYNSELLDVYCSQCCKKINLLNDLEARLKNLKANSPNRKISSTAFGRQLMHSSNFSSSNGSTEDLFRDSIDSCDNDITEKVSFLEKKVTELENDSLTNGDLKSKLKQENTQLVHRVHELEEMVKDQETTAEQALEEEARRHREAYGKLEREKATEVELLNARVQQLEEENTELRTTVTRLKSQTEKLDEERQRMSDRLEDTSLRLKDEMDLYKRMMDKLRQNRLEFQKEREATQELIEDLRKELEHLQMYKLDCERPGRGRSASSGLGEFNARAREVELEHEVKRLKQENYKLRDQNDDLNGQILSLSLYEAKNLFAAQTKAQSLAAEIDTASRDELMEALKEQEEINFRLRQYMDKIILAILDHNPSILEIKH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86YS3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y60 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot |
研究背景
Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. In case of infection by HCMV (human cytomegalovirus), may participate in egress of the virus out of nucleus; this function is independent of ARF6.
Endosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Recycling endosome membrane>Peripheral membrane protein. Cleavage furrow. Midbody. Cytoplasmic vesicle.
Note: Recruited to the cleavage furrow and the midbody during cytokinesis.
Present at high level in testis (at protein level). Weakly expressed in other tissues.
Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Forms a heterooligomeric complex with RAB11FIP2, RAB11FIP3 and RAB11FIP5. Interacts with ECPAS.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein gM/UL100.
The RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B).
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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