RAB3IP Antibody - #AF9176
| 製品: | RAB3IP Antibody |
| カタログ: | AF9176 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAB3IP |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 53kDa; 53kD(Calculated). |
| ユニプロット: | Q96QF0 |
| RRID: | AB_2843366 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9176, RRID:AB_2843366.
折りたたみ／展開
Gtpat12; Rab-3A-interacting protein; RAB3A interacting protein; RAB3A interacting protein (rabin3); RAB3A interacting protein; RAB3A-interacting protein; RAB3I_HUMAN; Rab3ip; Rabin 3; Rabin-3; RABIN3; RABIN3, rat, homolog of; Rabin8; SSX2 interacting protein; SSX2 interacting protein; SSX2-interacting protein;
免疫原
Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
- Q96QF0 RAB3I_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLKKMKGLSYDEAFAMANDPLEGFHEVNLASPTSPDLLGVYESGTQEQTTSPSVIYRPHPSALSSVPIQANALDVSELPTQPVYSSPRRLNCAEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANIKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLPGGKTPFKKGHTRNKSTSSAMSGSHQDLSVIQPIVKDCKEADLSLYNEFRLWKDEPTMDRTCPFLDKIYQEDIFPCLTFSKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKSCKHRIKLGDSSNYYYISPFCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96QF0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y85 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| T277 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Q9Y2H1 (STK38L) , Q15208 (STK38) | Uniprot |
| T289 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K383 | Acetylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| K462 | Methylation | Uniprot | |
| Y471 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
Interacts with the N-terminal region of SSX2. Interacts with the GDP-bound forms of RAB8A and RAB8B. The interaction with RAB8A is prevented by phosphorylation of RAB8A at 'Thr-72'. Interacts with the GDP-bound forms of RAB3A and RAB3D. Interacts with DCDC1.
Belongs to the SEC2 family.
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