RCC2 Antibody - #AF9180
製品: | RCC2 Antibody |
カタログ: | AF9180 |
タンパク質の説明: | Rabbit polyclonal antibody to RCC2 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 56kDa; 56kD(Calculated). |
ユニプロット: | Q9P258 |
RRID: | AB_2843370 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9180, RRID:AB_2843370.
折りたたみ/展開
KIAA1470; Protein RCC2; RCC 2; RCC1 like protein TD 60; RCC1-like protein TD-60; RCC2; RCC2_HUMAN; Regulator of chromosome condensation 2; TD60; Telophase disk protein of 60 kDa;
免疫原
- Q9P258 RCC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRKKAAAAAWEEPSSGNGTARAGPRKRGGPAGRKRERPERCSSSSGGGSSGDEDGLELDGAPGGGKRAARPATAGKAGGAAVVITEPEHTKERVKLEGSKCKGQLLIFGATNWDLIGRKEVPKQQAAYRNLGQNLWGPHRYGCLAGVRVRTVVSGSCAAHSLLITTEGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACGRNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGCPEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9P258 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K4 | Methylation | Uniprot | |
K5 | Sumoylation | Uniprot | |
K5 | Ubiquitination | Uniprot | |
S15 | Phosphorylation | Uniprot | |
S16 | Phosphorylation | Uniprot | |
T20 | Phosphorylation | Uniprot | |
R22 | Methylation | Uniprot | |
K27 | Ubiquitination | Uniprot | |
R28 | Methylation | Uniprot | |
C42 | S-Nitrosylation | Uniprot | |
S43 | Phosphorylation | Uniprot | |
S44 | Phosphorylation | Uniprot | |
S45 | Phosphorylation | Uniprot | |
S46 | Phosphorylation | Uniprot | |
S50 | Phosphorylation | Uniprot | |
S51 | Phosphorylation | Uniprot | |
T74 | Phosphorylation | Uniprot | |
K77 | Ubiquitination | Uniprot | |
T91 | Phosphorylation | Uniprot | |
K92 | Acetylation | Uniprot | |
K92 | Sumoylation | Uniprot | |
K92 | Ubiquitination | Uniprot | |
K96 | Acetylation | Uniprot | |
K96 | Sumoylation | Uniprot | |
K124 | Ubiquitination | Uniprot | |
Y142 | Phosphorylation | Uniprot | |
K170 | Ubiquitination | Uniprot | |
R176 | Methylation | Uniprot | |
K179 | Ubiquitination | Uniprot | |
S200 | Phosphorylation | Uniprot | |
Y276 | Phosphorylation | Uniprot | |
S277 | Phosphorylation | Uniprot | |
Y283 | Phosphorylation | Uniprot | |
S290 | Phosphorylation | Uniprot | |
K293 | Acetylation | Uniprot | |
K293 | Methylation | Uniprot | |
K293 | Ubiquitination | Uniprot | |
T319 | Phosphorylation | Uniprot | |
K320 | Acetylation | Uniprot | |
K320 | Ubiquitination | Uniprot | |
T342 | Phosphorylation | Uniprot | |
S347 | Phosphorylation | Uniprot | |
K349 | Acetylation | Uniprot | |
K349 | Ubiquitination | Uniprot | |
Y359 | Phosphorylation | Uniprot | |
K368 | Acetylation | Uniprot | |
K368 | Ubiquitination | Uniprot | |
K377 | Acetylation | Uniprot | |
K377 | Ubiquitination | Uniprot | |
K422 | Acetylation | Uniprot | |
K422 | Ubiquitination | Uniprot | |
K468 | Ubiquitination | Uniprot | |
T473 | Phosphorylation | Uniprot | |
K479 | Ubiquitination | Uniprot | |
K513 | Acetylation | Uniprot | |
K513 | Methylation | Uniprot | |
K513 | Ubiquitination | Uniprot | |
Y517 | Phosphorylation | Uniprot | |
R520 | Methylation | Uniprot | |
T521 | Phosphorylation | Uniprot |
研究背景
Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA. Interferes with the activation of RAC1 by guanine nucleotide exchange factors. Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions. Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro).
Nucleus>Nucleolus. Nucleus. Cytoplasm>Cytoskeleton. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle. Chromosome. Midbody. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378). Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455). Colocalizes with RAC1 at the cell membrane (PubMed:25074804).
Interacts with RAC1. Interacts with nucleotide-free and with GDP and GTP-bound forms of RAC1, with a slight preference for GDP-bound RAC1. Binds preferentially to the nucleotide-free form of RAC1. Interacts with CORO1C. Interacts with microtubules.
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