製品: RCC2 Antibody
カタログ: AF9180
タンパク質の説明: Rabbit polyclonal antibody to RCC2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 56kDa; 56kD(Calculated).
ユニプロット: Q9P258
RRID: AB_2843370

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(92%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
RCC2 Antibody detects endogenous levels of total RCC2.
RRID:
AB_2843370
引用形式: Affinity Biosciences Cat# AF9180, RRID:AB_2843370.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

KIAA1470; Protein RCC2; RCC 2; RCC1 like protein TD 60; RCC1-like protein TD-60; RCC2; RCC2_HUMAN; Regulator of chromosome condensation 2; TD60; Telophase disk protein of 60 kDa;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MPRKKAAAAAWEEPSSGNGTARAGPRKRGGPAGRKRERPERCSSSSGGGSSGDEDGLELDGAPGGGKRAARPATAGKAGGAAVVITEPEHTKERVKLEGSKCKGQLLIFGATNWDLIGRKEVPKQQAAYRNLGQNLWGPHRYGCLAGVRVRTVVSGSCAAHSLLITTEGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACGRNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGCPEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
92
Xenopus
91
Pig
67
Bovine
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P258 基板として

Site PTM Type Enzyme
K4 Methylation
K5 Sumoylation
K5 Ubiquitination
S15 Phosphorylation
S16 Phosphorylation
T20 Phosphorylation
R22 Methylation
K27 Ubiquitination
R28 Methylation
C42 S-Nitrosylation
S43 Phosphorylation
S44 Phosphorylation
S45 Phosphorylation
S46 Phosphorylation
S50 Phosphorylation
S51 Phosphorylation
T74 Phosphorylation
K77 Ubiquitination
T91 Phosphorylation
K92 Acetylation
K92 Sumoylation
K92 Ubiquitination
K96 Acetylation
K96 Sumoylation
K124 Ubiquitination
Y142 Phosphorylation
K170 Ubiquitination
R176 Methylation
K179 Ubiquitination
S200 Phosphorylation
Y276 Phosphorylation
S277 Phosphorylation
Y283 Phosphorylation
S290 Phosphorylation
K293 Acetylation
K293 Methylation
K293 Ubiquitination
T319 Phosphorylation
K320 Acetylation
K320 Ubiquitination
T342 Phosphorylation
S347 Phosphorylation
K349 Acetylation
K349 Ubiquitination
Y359 Phosphorylation
K368 Acetylation
K368 Ubiquitination
K377 Acetylation
K377 Ubiquitination
K422 Acetylation
K422 Ubiquitination
K468 Ubiquitination
T473 Phosphorylation
K479 Ubiquitination
K513 Acetylation
K513 Methylation
K513 Ubiquitination
Y517 Phosphorylation
R520 Methylation
T521 Phosphorylation

研究背景

機能:

Multifunctional protein that may effect its functions by regulating the activity of small GTPases, such as RAC1 and RALA. Required for normal progress through the cell cycle, both during interphase and during mitosis. Required for the presence of normal levels of MAD2L1, AURKB and BIRC5 on inner centromeres during mitosis, and for normal attachment of kinetochores to mitotic spindles. Required for normal organization of the microtubule cytoskeleton in interphase cells. Functions as guanine nucleotide exchange factor (GEF) for RALA. Interferes with the activation of RAC1 by guanine nucleotide exchange factors. Prevents accumulation of active, GTP-bound RAC1, and suppresses RAC1-mediated reorganization of the actin cytoskeleton and formation of membrane protrusions. Required for normal cellular responses to contacts with the extracellular matrix of adjacent cells, and for directional cell migration in response to a fibronectin gradient (in vitro).

細胞の位置付け:

Nucleus>Nucleolus. Nucleus. Cytoplasm>Cytoskeleton. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle. Chromosome. Midbody. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378). Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455). Colocalizes with RAC1 at the cell membrane (PubMed:25074804).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with RAC1. Interacts with nucleotide-free and with GDP and GTP-bound forms of RAC1, with a slight preference for GDP-bound RAC1. Binds preferentially to the nucleotide-free form of RAC1. Interacts with CORO1C. Interacts with microtubules.

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