Separase Antibody - #AF9195
(2)
| 製品: | Separase Antibody |
| カタログ: | AF9195 |
| タンパク質の説明: | Rabbit polyclonal antibody to Separase |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Sheep, Rabbit, Dog |
| 分子量: | 230kDa; 233kD(Calculated). |
| ユニプロット: | Q14674 |
| RRID: | AB_2843385 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human
予測:
Sheep(80%), Rabbit(90%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
Separase Antibody detects endogenous levels of total Separase.
RRID:
AB_2843385
引用形式: Affinity Biosciences Cat# AF9195, RRID:AB_2843385.
引用形式: Affinity Biosciences Cat# AF9195, RRID:AB_2843385.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q14674 ESPL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
90
Dog
90
Sheep
80
Pig
0
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14674 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K562 | Acetylation | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| Y719 | Phosphorylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K755 | Ubiquitination | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| Y973 | Phosphorylation | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| K1016 | Ubiquitination | Uniprot | |
| K1020 | Ubiquitination | Uniprot | |
| S1073 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| T1111 | Phosphorylation | Uniprot | |
| S1122 | Phosphorylation | Uniprot | |
| T1123 | Phosphorylation | Uniprot | |
| S1126 | Phosphorylation | P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
| S1153 | Phosphorylation | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| T1210 | Phosphorylation | Uniprot | |
| K1252 | Ubiquitination | Uniprot | |
| S1305 | Phosphorylation | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| S1316 | Phosphorylation | Uniprot | |
| K1322 | Ubiquitination | Uniprot | |
| S1325 | Phosphorylation | Uniprot | |
| K1336 | Ubiquitination | Uniprot | |
| R1341 | Methylation | Uniprot | |
| T1346 | Phosphorylation | Uniprot | |
| T1363 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1396 | Phosphorylation | Uniprot | |
| S1399 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1444 | Phosphorylation | Uniprot | |
| S1455 | Phosphorylation | Uniprot | |
| S1475 | Phosphorylation | Uniprot | |
| S1501 | Phosphorylation | P17612 (PRKACA) , Q9UQM7 (CAMK2A) | Uniprot |
| S1508 | Phosphorylation | Uniprot | |
| S1513 | Phosphorylation | Uniprot | |
| K1518 | Ubiquitination | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| S1538 | Phosphorylation | Uniprot | |
| S1539 | Phosphorylation | Uniprot | |
| S1545 | Phosphorylation | Uniprot | |
| S1552 | Phosphorylation | Uniprot | |
| Y1614 | Phosphorylation | Uniprot | |
| S1649 | Phosphorylation | Uniprot | |
| S1660 | Phosphorylation | Uniprot | |
| K1692 | Ubiquitination | Uniprot | |
| K1732 | Ubiquitination | Uniprot | |
| K1747 | Ubiquitination | Uniprot | |
| S1752 | Phosphorylation | Uniprot | |
| K1762 | Ubiquitination | Uniprot | |
| S1768 | Phosphorylation | Uniprot | |
| S1769 | Phosphorylation | Uniprot | |
| T1771 | Phosphorylation | Uniprot | |
| K1796 | Ubiquitination | Uniprot | |
| K1803 | Ubiquitination | Uniprot | |
| S1820 | Phosphorylation | Uniprot | |
| K1832 | Ubiquitination | Uniprot | |
| T1884 | Phosphorylation | Uniprot | |
| S1887 | Phosphorylation | Uniprot | |
| S1889 | Phosphorylation | Uniprot | |
| K1901 | Ubiquitination | Uniprot | |
| K1994 | Ubiquitination | Uniprot | |
| K2103 | Ubiquitination | Uniprot | |
| Y2104 | Phosphorylation | Uniprot | |
| S2118 | Phosphorylation | Uniprot |
研究背景
機能:
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
PTMs:
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.
細胞の位置付け:
Cytoplasm. Nucleus.
サブユニット構造:
Interacts with PTTG1. Interacts with RAD21.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
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