StIp1 Antibody - #AF9204

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製品: StIp1 Antibody
カタログ: AF9204
タンパク質の説明: Rabbit polyclonal antibody to StIp1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 93kDa; 93kD(Calculated).
ユニプロット: Q6IA86
RRID: AB_2843394

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 100ul $280 在庫あり
 200ul $350 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(89%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
StIp1 Antibody detects endogenous levels of total StIp1.
RRID:
AB_2843394
引用形式: Affinity Biosciences Cat# AF9204, RRID:AB_2843394.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AU023723; Elongation protein 2 homolog (S. cerevisiae); Elongator acetyltransferase complex subunit 2; Elongator complex protein 2; elongator protein 2; ELP2; ELP2_HUMAN; Epl2; FLJ10879; SHINC 2; SHINC-2; SHINC2; signal transducer and activator of transcription 3 interacting protein 1; signal transducer and activator of transcription interacting protein 1; STAT3 interacting protein 1; Stat3 interacting protein; STAT3-interacting protein 1; STATIP 1; STATIP1; STATIP1 protein; StIP; StIP1;

免疫原

免疫原:
Uniprot:

Q6IA86(ELP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
ELP2(55250)
タンパク質配列:
MVAPVLETSHVFCCPNRVRGVLNWSSGPRGLLAFGTSCSVVLYDPLKRVVVTNLNGHTARVNCIQWICKQDGSPSTELVSGGSDNQVIHWEIEDNQLLKAVHLQGHEGPVYAVHAVYQRRTSDPALCTLIVSAAADSAVRLWSKKGPEVMCLQTLNFGNGFALALCLSFLPNTDVPILACGNDDCRIHIFAQQNDQFQKVLSLCGHEDWIRGVEWAAFGRDLFLASCSQDCLIRIWKLYIKSTSLETQDDDNIRLKENTFTIENESVKIAFAVTLETVLAGHENWVNAVHWQPVFYKDGVLQQPVRLLSASMDKTMILWAPDEESGVWLEQVRVGEVGGNTLGFYDCQFNEDGSMIIAHAFHGALHLWKQNTVNPREWTPEIVISGHFDGVQDLVWDPEGEFIITVGTDQTTRLFAPWKRKDQSQVTWHEIARPQIHGYDLKCLAMINRFQFVSGADEKVLRVFSAPRNFVENFCAITGQSLNHVLCNQDSDLPEGATVPALGLSNKAVFQGDIASQPSDEEELLTSTGFEYQQVAFQPSILTEPPTEDHLLQNTLWPEVQKLYGHGYEIFCVTCNSSKTLLASACKAAKKEHAAIILWNTTSWKQVQNLVFHSLTVTQMAFSPNEKFLLAVSRDRTWSLWKKQDTISPEFEPVFSLFAFTNKITSVHSRIIWSCDWSPDSKYFFTGSRDKKVVVWGECDSTDDCIEHNIGPCSSVLDVGGAVTAVSVCPVLHPSQRYVVAVGLECGKICLYTWKKTDQVPEINDWTHCVETSQSQSHTLAIRKLCWKNCSGKTEQKEAEGAEWLHFASCGEDHTVKIHRVNKCAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
89
Chicken
88
Xenopus
75
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6IA86 基板として

Site PTM Type Enzyme
Y117 Phosphorylation
K241 Ubiquitination
K256 Acetylation
K256 Ubiquitination
K314 Ubiquitination
K419 Ubiquitination
K421 Ubiquitination
K442 Ubiquitination
K459 Ubiquitination
S519 Phosphorylation
Y532 Phosphorylation
K579 Ubiquitination
K587 Ubiquitination
Y752 Phosphorylation
K756 Ubiquitination
K788 Ubiquitination
K797 Ubiquitination
K817 Ubiquitination
K823 Ubiquitination

研究背景

機能:

Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with STAT3 and JAKs (By similarity).

タンパク質ファミリー:

Belongs to the WD repeat ELP2 family.

参考文献

1). Stress-induced phosphoprotein 1 restrains spinal cord ischaemia-reperfusion injury by modulating NF-κB signalling. Journal of Cellular and Molecular Medicine, 2021 (PubMed: 34734476) [IF=5.3]

Application: WB    Species: Rat    Sample: spinal cord

FIGURE 1 STIP1 expression increases firstly and then decreases in rats after SCII treatment. A, The rats undergo ischaemia by clamping abdominal aorta for 1 h and reperfusion for 24 h; B, Western blotting detects STIP1 expression in rat spinal cord specimens in absence or presence of SCII treatment; C, Immunohistochemical staining detects STIP1 expression in rat spinal cord specimens. The brown represents STIP1, and the STIP1-postive cells are indicated by arrows. Scale bar =50 μm. SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1. **p < 0.01, ***p < 0.001 versus the Sham group

Application: IF/ICC    Species: Rat    Sample: spinal cord

FIGURE 3 STIP1 ameliorates ischaemia/reperfusion-induced inflammation in rat spinal cord. A, Immunohistochemical staining determines the expression and location of Iba-1 and STIP1. The arrows indicate co-location of STIP1 and Iba-1. Scale bar =50 μm; B, ELISA detects TNF-α and IL-6 secretion in rat spinal cord specimens following SCII treatment and STIP1 overexpression; C, Western blotting determines IκBβ expression in rat spinal cord specimens; D, Western blotting determines the cytoplasmic and nuclear expression of NF-κB p65. NF-κB, nuclear factor kappa B; SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1; TNF, tumour necrosis factor. ***p < 0.001 versus the Sham group; #p < 0.05, ###p < 0.001 versus the SCII + LV group

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