TUTase Antibody - #AF9219
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF9219, RRID:AB_2843409.
折りたたみ／展開
alpha regulated poly(A) polymerase; nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I; nuclear speckle targeted PIPK1A regulated poly(A) polymerase; PAP associated domain containing 2; PAPD2; poly(A) polymerase associated domain containing 2; RBM21; RNA binding motif protein 21; RNA-binding motif protein 21; RNA-binding protein 21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; STARPAP; STPAP_HUMAN; terminal uridylyl transferase 1, U6 snRNA specific; TUT1; TUTase; U6 snRNA specific terminal uridylyltransferase 1; U6 snRNA-specific terminal uridylyltransferase 1; U6 TUTase; U6-TUTase; URLC6;
免疫原
- Q9H6E5 STPAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVDSDVESLPRGGFRCCLCHVTTANRPSLDAHLGGRKHRHLVELRAARKAQGLRSVFVSGFPRDVDSAQLSEYFLAFGPVASVVMDKDKGVFAIVEMGDVGAREAVLSQSQHSLGGHRLRVRPREQKEFQSPASKSPKGAAPDSHQLAKALAEAADVGAQMIKLVGLRELSEAERQLRSLVVALMQEVFTEFFPGCVVHPFGSSINSFDVHGCDLDLFLDLGDLEEPQPVPKAPESPSLDSALASPLDPQALACTPASPPDSQPPASPQDSEALDFETPSSSLAPQTPDSALASETLASPQSLPPASPLLEDREEGDLGKASELAETPKEEKAEGAAMLELVGSILRGCVPGVYRVQTVPSARRPVVKFCHRPSGLHGDVSLSNRLALHNSRFLSLCSELDGRVRPLVYTLRCWAQGRGLSGSGPLLSNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEGEQVEVDGWDCSFPRDASRLEPSINVEPLSSLLAQFFSCVSCWDLRGSLLSLREGQALPVAGGLPSNLWEGLRLGPLNLQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYCRSLQYQRRSSRGRDWGLLPLLQPSSPSSLLSATPIPLPLAPFTQLTAALVQVFREALGCHIEQATKRTRSEGGGTGESSQGGTSKRLKVDGQKNCCEEGKEEQQGCAGDGGEDRVEEMVIEVGEMVQDWAMQSPGQPGDLPLTTGKHGAPGEEGQPSHAALAERGPKGHEAAQEWSQGEAGKGASLPSSASWRCALWHRVWQGRRRARRRLQQQTKEGAGGGAGTRAGWLATEAQVTQELKGLSGGEERPETEPLLSFVASVSPADRMLTVTPLQDPQGLFPDLHHFLQVFLPQAIRHLK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H6E5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S10 | Phosphorylation | Uniprot | |
| R13 | Methylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| T649 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| K659 | Acetylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| S750 | Phosphorylation | Q13535 (ATR) | Uniprot |
| K790 | Ubiquitination | Uniprot |
研究背景
Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
Phosphorylated by CK1 in the proline-rich (Pro-rich) region.
Nucleus>Nucleolus. Nucleus speckle.
Widely expressed.
Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A; interaction.
Belongs to the DNA polymerase type-B-like family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.