MMP1 Antibody - #AF0209

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製品: MMP1 Antibody
カタログ: AF0209
タンパク質の説明: Rabbit polyclonal antibody to MMP1
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 54kDa; 54kD(Calculated).
ユニプロット: P03956
RRID: AB_2833396

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(88%), Bovine(88%), Horse(100%), Sheep(94%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
MMP1 Antibody detects endogenous levels of total MMP1.
RRID:
AB_2833396
引用形式: Affinity Biosciences Cat# AF0209, RRID:AB_2833396.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

27 kDa interstitial collagenase; CLG; CLGN; collagenase, fibroblast; collagenase, interstitial; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP-1; MMP1; MMP1_HUMAN; OTTHUMP00000045866;

免疫原

免疫原:

A synthesized peptide derived from human MMP1, corresponding to a region within C-terminal amino acids.

Uniprot:

P03956(MMP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
MMP1(4312)
タンパク質の説明:
MMP1 Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.
タンパク質配列:
MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Sheep
94
Pig
88
Bovine
88
Dog
69
Chicken
53
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

PTMs:

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

研究領域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

参考文献

1). Long-term simulated microgravity fosters carotid aging-like changes via Piezo1. Cardiovascular research, 2024 (PubMed: 38271270) [IF=10.2]
2). N6-methyladenosine Reader IGF2BP2-modified HMMR Promotes Non-small Cell Lung Cancer Metastasis via Interaction with MAP4K4. International journal of biological sciences, 2025 (PubMed: 39990663) [IF=8.2]
3). Brusatol Inhibits Proliferation and Invasion of Glioblastoma by Down-Regulating the Expression of ECM1. Frontiers in pharmacology, 2021 (PubMed: 34970146) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 6. The effect of ECM1 on invasion-related biomarkers. (A) Cells were transfected with siControl or siECM1 for 24–48 h and subjected to immunoblot analysis for measuring the expression of indicated proteins. (B) Cells were infected with pLV-vector (Control) or pLV-ECM1 (ECM1) for 24–48 h and then subjected to immunoblot analysis for measuring the expression of indicated proteins. (C) Immunoblot analyses showing ECM1, MMP2, TIMP2, MMP1, TIMP1 and MMP9 expression levels in xenograft tumors. The blots shown here were representatives of the average of five subcutaneously transplanted tumors in nude mice. (D) Representative images of IHC staining showed that Bru decreased the levels of ECM1 in subcutaneously transplanted xenograft tumors ().
4). Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway. RESPIRATORY RESEARCH, 2018 (PubMed: 29871641) [IF=4.7]

Application: IHC    Species: human    Sample: lung

Fig. 6| PTL attenuates the BLM-induced expression of EMT-related protein. a Representative immunohistochemical staining of lung sections showing cadherin, vimentin, MMP1, α-SMA and Col-1 staining.
5). Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification. Drug Design Development and Therapy, 2021 (PubMed: 34616145) [IF=4.7]

Application: IHC    Species: Mice    Sample: colon tissues

Figure 9 Representative immunohistochemical staining with MMP1, MMP3, MMP7, MMP9 and MMP12 in colon tissues in mice treated with DSS. (A) Immunohistochemical staining for MMP1, MMP3, MMP7, MMP9 and MMP12 in normal, model and DSS+HTD group. (B) Statistical analysis of relative immunohistochemical staining intensity for MMP1, MMP3, MMP7, MMP9 and MMP12 in normal, model and DSS+HTD group.*P < 0.05, **P < 0.01 and ***P < 0.001 versus model group.
6). Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro. Bioengineered, 2022 (PubMed: 35094658) [IF=4.2]

Application: WB    Species: Rat    Sample: SW1353 cells and chondrocytes

Figure 4. TB-II inhibited the production of cartilage degrading enzymes in IL-1β-treated SW1353 cells and chondrocytes. The mRNA expression of (a) MMP-1, (b) MMP-3 and (c) MMP-13 were detected by qRT-PCR. (d-g) The protein level of MMP-1, MMP-3, and MMP-13 were detected by Western blot. GAPDH was conducted as a loading control. One-way ANOVA, ##p < 0.01, compared with control cells; $p < 0.05, $$p < 0.01, ns: not-significant, compared with IL-1β-treated cells.
7). Milk-derived exosome-loaded SS31 as a novel strategy to mitigate UV-induced photodamage in skin. Journal of photochemistry and photobiology. B, Biology, 2025 (PubMed: 39970726) [IF=3.9]
8). Overexpression of SIRT6 regulates NRF2/HO-1 and NF-κB signaling pathways to alleviate UVA-induced photoaging in skin fibroblasts. Journal of photochemistry and photobiology. B, Biology, 2023 (PubMed: 37897855) [IF=3.9]
9). Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32737772) [IF=3.5]

Application: WB    Species: rat    Sample: chondrocytes

FIGURE 2 |e) Western blot analysis (right panel) and band intensity analysis (left panel) of the protein levels of Survivin, Mmp1, and Mmp13 in Control+Vehicle, Vector+IL-1β, and oeUSP49 +IL-1β rat chondro-cytes. ***P<0.001 versus the Vector or Control+Vehicle group; ## and ### indicate P values less than 0.01 and 0.001,respectively, versus the Vec-tor+IL-1β group
10). Human embryonic stem cells exert antitumor effects on prostate cancer cells in a co-culture microenvironment. Frontiers in oncology, 2023 (PubMed: 37313467) [IF=3.5]
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