SLC1A5 Antibody - #AF6610

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製品: SLC1A5 Antibody
カタログ: AF6610
タンパク質の説明: Rabbit polyclonal antibody to SLC1A5
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 56 kDa; 57kD(Calculated).
ユニプロット: Q15758
RRID: AB_2843431

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
SLC1A5 Antibody detects endogenous levels of total SLC1A5.
RRID:
AB_2843431
引用形式: Affinity Biosciences Cat# AF6610, RRID:AB_2843431.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ASCT2; AAAT; AAAT_HUMAN; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; Neutral amino acid transporter B(0); R16; RD114/simian type D retrovirus receptor; RDR; RDRC; SLC1A5; Sodium dependent neutral amino acid transporter type 2; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 (neutral amino acid transporter), member 5; Solute carrier family 1 member 5;

免疫原

免疫原:
Uniprot:

Q15758(AAAT_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC1A5(6510)
発現特異性:
Q15758 AAAT_HUMAN:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

タンパク質の説明:
Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
タンパク質配列:
MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15758 基板として

Site PTM Type Enzyme
M1 Acetylation
S9 Phosphorylation
K10 Ubiquitination
S27 Phosphorylation
Y38 Phosphorylation
K178 Ubiquitination
S183 Phosphorylation
S194 Phosphorylation
S198 Phosphorylation
N212 N-Glycosylation
K247 Ubiquitination
K372 Ubiquitination
S493 Phosphorylation
T494 Phosphorylation
K502 Ubiquitination
S503 Phosphorylation
K522 Sumoylation
K522 Ubiquitination
Y524 Phosphorylation
R525 Methylation
T532 Phosphorylation
S535 Phosphorylation
K537 Acetylation
K537 Ubiquitination
S539 Phosphorylation

研究背景

機能:

Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development.

(Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.

(Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.

(Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

サブユニット構造:

Homotrimer (Probable). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization.

タンパク質ファミリー:

Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.

研究領域

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Digestive system > Protein digestion and absorption.

参考文献

1). Tripartite motif containing 33 demonstrated anticancer effect by degrading c‑Myc: Limitation of glutamine metabolism and proliferation in endometrial carcinoma cells. International journal of oncology, 2023 (PubMed: 37859625) [IF=5.2]

Application: WB    Species: Human    Sample: EC cell

Figure 4 TRIM33 overexpression inhibits the glutamine metabolism in HEC-1-A and AN3CA cells in vitro. (A) Glutamine uptake and (B) intracellular glutamate production of TRIM33-silenced or -overexpressed EC cells were analyzed using the commercial kits. (C and D) Reverse transcription-quantitative PCR and western blotting were used to detect the expression of SLC1A5 and GLS in EC cells. *P
2). Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1. Pharmaceutical biology, 2024 (PubMed: 38571483) [IF=3.8]

Application: WB    Species: Human    Sample: CCAs

Figure 6. The extent of apoptosis, glutathione (GSH) synthesis, and the expression of DNA damage-related proteins were assessed in cholangiocarcinoma cells (CCAs) subjected to YAP1 knockdown or YAP1 overexpression. Western blot was used to measure protein levels (n = 3). With cisplatin incubation, YAP1 knockdown and 1 mg/mL babaodan (BBD) treatment decreased the (a) Bcl-2 level and (b) increased bax level, while the change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In the CCAs dealing with cisplatin, the expression levels of (d) p-YAP1/YAP1, (e) ATF4, and (f) SLC1A5 were decreased by YAP1 knockdown and BBD treatment. Additionally, the (g) γH2Ax level was increased and (h) the ERCC1 level was inhibited by YAP1 knockdown and BBD treatment. YAP1 overexpression antagonized the effect of BBD on these proteins. Representative protein bands are shown in (i), (j), and (k). (mean ± standard deviation) +p 
3). Dysfunction of Cytotoxic T Lymphocyte Induced by Hepatoma Cells through the Gln-GLS2-Endoplasmic Reticulum Stress Pathway. Frontiers in Bioscience-Landmark, 2022 (PubMed: 36042180)

Application: WB    Species: Mouse    Sample: cytotoxic T lymphocytes (CTLs) and hepatoma cells

Fig. 4. Glutamine (Gln) utilization in cytotoxic T lymphocytes (CTLs) and hepatoma cells. (A) Western blots were used to detect the protein expression levels of GLS1, GLS2, and SLC1A5 in the different groups. (B) After Gln deprivation, protein expression levels of GLS1, GLS2, and SLC1A5 in both CTLs and hepatoma cells were assessed by western blots.

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