製品: mCherry-Tag Antibody
カタログ: T0090
タンパク質の説明: Mouse monoclonal antibody to mCherry-Tag
アプリケーション: WB IF/ICC ELISA
反応性: All
ユニプロット:
RRID: AB_2843441

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:1000-1:5000, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
All
クローナリティ:
Monoclonal [T235]
特異性:
The mCherry tag antibody can recognize mCherry and mCherry-tag fusion proteins.
RRID:
AB_2843441
引用形式: Affinity Biosciences Cat# T0090, RRID:AB_2843441.
コンジュゲート:
Unconjugated.
精製:
affinity purification.
保存:
Store at -20°C. Stable for one year from the date of shipment.1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

mCherry,mCherry antibody

参考文献

1). YY1 Oligomerization Is Regulated by Its OPB Domain and Competes with Its Regulation of Oncoproteins. Cancers, 2022 (PubMed: 35406384) [IF=5.2]

Application: WB    Species: Human    Sample:

Figure 2. Identification of the binding sites responsible for YY1 dimerization by co-immunoprecipitation (co-IP). (A) Diagrams of HA- and Flag-tagged YY1 wt and its mutant proteins used for co-IP studies. (B,C) Co-IP studies to determine the binding sites responsible for YY1 dimerization. HA-YY1 wt (B) or its ΔZF mutant (C) expression plasmid was individually cotransfected with Flag-EGFP-YY1 wt and mutant expression vectors, as well as an empty vector (EV). Cell lysates were co-IPed by a Flag antibody, and HA and Flag antibodies were used in Western blot analyses. (D) Schematic diagram of a predicted YY1 dimerization mode. (E) Effects of the OPB on intermolecular interaction of YY1. Different amounts of mCherry-cont, mCherry-OPB and mCherry plasmids were individually cotransfected with Flag-EGFP-YY1(ΔZF) and HA-YY1(ΔZF) expression vectors, followed by co-IP of the Flag antibody. HA, mCherry and Flag antibodies were used in Western blot analyses.

2). RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus. PLOS Genetics, 2023 (PubMed: 36735729) [IF=4.5]

3). CRMP2 modulates mossy fiber sprouting in dentate gyrus of pilocarpine induced rat model of epilepsy. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022 (PubMed: 35334412) [IF=3.1]

4). Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice. Aging (Albany NY), 2021 (PubMed: 33411679) [IF=0.4]

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