MeCP2 Antibody - #AF9265

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製品: MeCP2 Antibody
カタログ: AF9265
タンパク質の説明: Rabbit polyclonal antibody to MeCP2
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Horse, Rabbit, Dog
分子量: 53 kDa.; 52kD(Calculated).
ユニプロット: P51608
RRID: AB_2843443

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MSDS
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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
MeCP2 Antibody detects endogenous levels of total MeCP2.
RRID:
AB_2843443
引用形式: Affinity Biosciences Cat# AF9265, RRID:AB_2843443.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;

免疫原

免疫原:
Uniprot:

P51608(MECP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MECP2(4204)
発現特異性:
P51608 MECP2_HUMAN:

Present in all adult somatic tissues tested.

タンパク質の説明:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
タンパク質配列:
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
75
Sheep
67
Bovine
62
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51608 基板として

Site PTM Type Enzyme
S13 Phosphorylation
S53 Phosphorylation
S70 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation Q9H2X6 (HIPK2)
S86 Phosphorylation
R115 Methylation
S116 Phosphorylation
K119 Methylation
Y120 Phosphorylation
K130 Ubiquitination
S149 Phosphorylation
T158 Phosphorylation
R162 Methylation
S164 Phosphorylation
S166 Phosphorylation
S178 Phosphorylation
T184 Phosphorylation
T203 Phosphorylation
S204 Phosphorylation
K210 Methylation
S216 Phosphorylation
K219 Acetylation
K223 Ubiquitination
T228 Phosphorylation
S229 Phosphorylation
K267 Methylation
S274 Phosphorylation
K285 Acetylation
T308 Phosphorylation
T311 Phosphorylation
S313 Phosphorylation
K321 Acetylation
T327 Phosphorylation
S332 Phosphorylation
K347 Methylation
S349 Phosphorylation
S355 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S360 Phosphorylation
S395 Phosphorylation
S423 Phosphorylation
S426 Phosphorylation
K449 Acetylation
T479 Phosphorylation
S486 Phosphorylation

研究背景

機能:

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

PTMs:

Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

細胞の位置付け:

Nucleus.
Note: Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present in all adult somatic tissues tested.

サブユニット構造:

Interacts with FNBP3 (By similarity). Interacts with CDKL5. Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells (By similarity). Interacts with NCOR2 (By similarity). Interacts with TBL1XR1; bridges interaction between MECP2 and NCOR1. Interacts with TBL1X; recruits TBL1X to the heterochromatin foci.

参考文献

1). The role of BDNF transcription in the antidepressant-like effects of 18β-glycyrrhetinic acid in a chronic social defeat stress model. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38851983) [IF=7.9]
2). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=4.3]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
3). CircMAPK1 promotes the proliferation and migration of vascular smooth muscle cells through miR-22-3p/methyl-CpG binding protein 2 axis. Nutrition, metabolism, and cardiovascular diseases : NMCD, 2021 (PubMed: 34059381) [IF=3.9]

Application: IF/ICC    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.The protein level of MECP2 was detected by Western blot (H) After indicated treatment,the expression and distribution of MECP2 was detected by immunofluorescence. Scale bar Z 50 mm. Each experiment was repeated for three times and the results were presented as mean SD. ))p < 0.01, )))p < 0.001. MECP2, methyl-CpG binding protein 2.

Application: WB    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.(E) The mRNA level of MECP2 was detected by quantitative real-time PCR (FeG)

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